Biolegend, 408214, PE anti-rat IgG2b Antibody, 500μg

The MRG2b-85 monoclonal antibody reacts with rat immunoglobulin G2b (IgG2b) in all tested rat strains (Lou, Lou/Ws1/M, Lewis, Wistar, DA, Sprague-Dawley). The MRG2b-85 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.
500μg
Verified Reactivity: Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: mixed rat Igs
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations: Banka AL, et al. 2023. Nat Commun. 14:2462. PubMed Liu H, et al. 2022. Cell Rep Med. 3:100660. PubMed
RRID: AB_2728432 (BioLegend Cat. No. 408213) AB_2749893 (BioLegend Cat. No. 408214)
Gene ID: NA
UniProt: View information about IgG2b on UniProt.org
Clone: MRG2b-85
Regulatory Status: RUO
Other Names: Immunoglobulin G2b
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.