Biolegend, 408302, Purified anti-FITC Antibody, 500μg

The FIT-22 antibody reacts with both fluorescein isothiocyanate (FITC) alone and FITC conjugated with antibody or streptavidin. When FIT-22 antibody is used in a three-step staining procedure or separation of FITC-antibody labeled cells, it does not quench FITC fluorescence or react with tested mouse and human cells. The FIT-22 antibody can be used for separation of FITC-antibody labeled cells or as a secondary step to amplify FITC conjugated primary antibody signals (i.e. biotinylated FIT-22 as secondary step, followed by Streptavidin-FITC).
500μg
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: FITC conjugated mouse IgG.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Yang LF, et al. 2022. Anal Chem. 94:12683. PubMed Rouers A, et al. 2021. Cell Rep Med. 2:100278. PubMed Hattori Y, et al. 2023. Cell Rep. 42:112092. PubMed Good Z, et al. 2019. Nat Biotechnol. 37:259. PubMed Chng MHY, et al. 2020. Immunity. 51(6):1119-1135.e5.. PubMed Mishra A, et al. 2021. Cell. 184(13):3394-3409.e20. PubMed
RRID: AB_528900 (BioLegend Cat. No. 408301) AB_528901 (BioLegend Cat. No. 408302)
Gene ID: NA
UniProt: View information about FITC on UniProt.org
Clone: FIT-22
Regulatory Status: RUO
Other Names: Fluorescein isothiocyanate
Isotype: Mouse IgG1, λ