Biolegend, 408608, PE anti-mouse IgM, 200μg

This antibody was raised by immunization with IgM a mAb TC31. It specifically reacts with mouse IgM of Igh-C a and the related haplotypes (c, f, g, h, and j). It has been reported that beads coated with MA-69 antibody can stimulate B cell proliferation.
200μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: ELISA as capture antibody or detection antibody, stimulation of B cell proliferation, and immunohistochemistry of acetone-fixed frozen sections.
Application References(PubMed link indicates BioLegend citation): Sieckmann D, et al. 1991. Hybridoma 10:121. Stall AM. 1996. Weir's Handbook of Experimental Immunology pp. 27.
Product Citations: Degn SE et al. 2017. Cell. 170(5):913-926 . PubMed Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed Firl DJ et al. 2018. eLife. 7 pii: e33051. PubMed Simoni L, et al. 2020. Cell Rep. 33:108330. PubMed Wan X, Thomas J, Unanue E 2016. J Exp Med. 213: 967 - 978. PubMed Smith LK, et al. 2021. Elife. 10:. PubMed Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
RRID: AB_940545 (BioLegend Cat. No. 408608)
Gene ID: 16019
UniProt: View information about IgMa on UniProt.org
Clone: MA-69
Regulatory Status: RUO
Other Names: Igh-6a
Isotype: Mouse (C57BL/6) IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.