Biolegend, 408916, PerCP/Cyanine5.5 anti-rat IgM Antibody, 100μg

The MRM-47 monoclonal antibody reacts with rat immunoglobulin M (IgM) in all tested rat strains (Lou, Lou/Ws1/M, Lewis, Wistar, DA, Sprague-Dawley). It does not react with other isotypes. The MRM-47 antibody can be used as a primary or secondary reagent for ELISA or immunofluorescent analysis.
100μg
Verified Reactivity: Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Mixed rat Igs
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
RRID: AB_2876734 (BioLegend Cat. No. 408915) AB_2876734 (BioLegend Cat. No. 408916)
Gene ID: 299357
UniProt: View information about IgM on UniProt.org
Clone: MRM-47
Regulatory Status: RUO
Other Names: Immunoglobulin M
Isotype: Mouse IgG
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.