Biolegend, 409506, PE anti-mouse Ig light chain κ Antibody, 200μg

The RMK-45 monoclonal antibody reacts with immunoglobulin light chain kappa in all tested mouse haplotype (Igh-a and b). It does not react with the lambda chain.
200μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: The RMK-12 monoclonal antibody may be used as a primary antibody for ELISA or as secondary for detection of mouse Ig kappa.
Product Citations: Pioli KT, et al. 2023. iScience. 26:106223. PubMed Zheng Z, et al. 2020. Cell Reports. 31(13):107819. PubMed Lazarian G, et al. 2021. Cancer Cell. 39(3):380-393.e8. PubMed
RRID: AB_2563580 (BioLegend Cat. No. 409505) AB_2563581 (BioLegend Cat. No. 409506)
Structure: Immunoglobulin family
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Protein Synthesis
Antigen References: 1. Ludwig TE, et al. 2006. Nat. Methods. 3:637. 2. Nguyen DH, et al. 2005. J. Immunol. 175:228. 3. Bardor M, et al. 2005. J. Biol. Chem. 280:4228. 4. Diaz SL, et al. 2009. PLoS One. 4:e4241.
Gene ID: 243469
UniProt: View information about Ig light chain kappa on UniProt.org
Clone: RMK-45
Regulatory Status: RUO
Other Names: Immunoglobulin light chain kappa
Isotype: Rat IgG
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.