Biolegend, 420516, Cell-Vive™ CD DMSO-Free Cryopreservation Solution, GMP, 100ml

Cell-Vive™ CD DMSO-Free Cryopreservation Solution, GMP is recommended to support human Mesenchymal Stem Cell (MSC) cryopreservation in a DMSO-free fashion. It is a chemically-defined formulation prepared without human or other animal-derived components. It is suitable for use in the cryopreservation of different MSC sources, without affecting relevant properties. When used under appropriate conditions, this product can preserve cell viability, expansion, phenotype, and differentiation capacity of MSCs. This GMP product is suggested for use in research and ex vivo cell processing. The benefits of this cryopreservation solution include: BioLegend Cell-Vive™ GMP cell culture products are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue- Engineered Products and Ph. Eur. Chapter 5.2.12 in a dedicated GMP facility compliant with ISO 13485:2016. Specifications and processes include:
100ml
Formulation: Chemically-defined. Cell-Vive™ CD DMSO-Free Cryopreservation Solution, GMP is a solution ready to use.
Endotoxin Level: < 1 EU/mL
Preparation: DMSO-Free, Chemically-Defined, no preservatives.
Storage & Handling: 4°C (2°C - 8°C)
Application: Cryopreservation of Human MSCs - Quality tested
Recommended Usage: Freeze 0.5-5 million cells per 1mL of Cell-Vive™ CD DMSO-Free Cryopreservation Solution, GMP
Application Notes: For cryopreservation, a range between 0.5-5 million cells per 1 mL of Cell-Vive™ CD DMSO-Free Cryopreservation Solution, GMP is recommended. The appearance of the product in liquid form is clear with a slight pink color. MSCs Cryopreservation protocol: Prepare a MSC suspension following an appropriate protocol. Centrifuge cells at 300 x g for 5 minutes to obtain a pellet. Carefully aspirate supernatant. Resuspend pelleted cells with Cell-Vive™ CD DMSO-Free Cryopreservation Solution, GMP and dispense the cell suspension into a cryovial. Immediately transfer the cryovial into a cell freezing container and store it at -70°C After 24-36 hours, transfer cryovials into a liquid nitrogen tank for long-term storage. Thawing MSCs protocol: Thaw cryovials containing cells by putting them in a 37°C water bath with very gently swirling. Thaw until only a small ice fragment is present. In a biosafety hood, add 1 mL of preferred cell culture media into the vial and then transfer content into a 15 mL conical tube containing 8 mL of cell culture media at room temperature. Gently mix. Centrifuge and remove the supernatant. Resuspend the cells at the desired density with cell culture media.
Disclaimer: BioLegend Cell-Vive™ GMP Cell Culture products are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.
Gene ID: NA
Other Names: Cryo Solution, DMSO-free, Cryopreservation media