Biolegend, 421301, Propidium Iodide Solution, 2ml

Propidium iodide (PI) is a fluorescent dye that binds to DNA. When excited by 488nm laser light, it can be detected with in the PE/Texas Red® channel with a bandpass filter 610/10. It is commonly used in evaluation of cell viability or DNA content in cell cycle analysis by flow cytometry.
2ml
Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide.
Concentration: 0.5 mg/ml
Storage & Handling: The solution should be stored undiluted between 2°C and 8°C, and protect from light. Caution: This solution contains hazardous material; handle with care.
Application: FC - Quality tested
Recommended Usage: The suggested use of this solution for viability staining is 10 µl per million cells in 0.5 ml/test, and incubate for 15 minutes at 4 °C before analysis. For Cell Cycle analysis, please see our Propidium Iodide Cell Cycle Staining Protocol. Caution: This solution is toxigenic and mutagenic; handle with care.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Propidium Iodide Solution can be used in evaluation of apoptosis, cell viability and cell cycle analysis by flow cytometry.
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Biology Area: Cell Biology, Cell Cycle/DNA Replication, Neuroscience
Gene ID: NA
Regulatory Status: RUO
Other Names: PI
Q: Why is washing not recommended after the addition 7-AAD or PI addition when assessing viability?
A: These dyes bind in equilibrium with DNA. Therefore, external dye concentration must be maintained during analysis and the dye should not be washed out.