Biolegend, 424401, True-Nuclear™ Transcription Factor Buffer Set, 120tests

BioLegend's True-Nuclear™ Transcription Factor Buffer Set has been specially formulated for intracellular staining with minimum effect on the surface fluorochrome staining. See the advantages of True-Nuclear™ Transcription Factor Buffer Set in this brief webinar: biolegend.com/true_nuclear_webinar . Caution : The True-Nuclear™ Transcription Factor Buffer Set contains paraformaldehyde, which is toxic and mutagenic. Please handle with caution. Wear gloves, lab coats, and necessary protection to avoid direct contact.
120tests
Storage & Handling: Store between 2°C and 8°C. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Prepare fresh Transcription Factor Fix working solution by diluting the 4X Fix Concentrate (1 part) with the Fix Diluent (3 parts). 1 mL of the 1X Fix working solution is needed for each tube. Prepare a 1X working solution of the Perm Buffer by diluting the 10X Perm Buffer with distilled water. 6.5 mL of 1X Perm Buffer is needed for each sample of tube. NOTE: The 10X Perm Buffer may have crystallization or precipitation when it is stored at 2-8°C; however, this is normal and does not affect the buffer's performance. If there is a heavy precipitation observed after dilution to 1X working solution, it can be filtered to clarify the solution.
Application Notes: Buffer Set Contents: True-Nuclear™ 4X Fix Concentrate (30 mL) True-Nuclear™ Fix Diluent (100 mL) True-Nuclear™ 10X Perm Buffer (100 mL)
Additional Product Notes: View more applications data using this product to stain Veri-Cells™ lyophilized control cells, to detect transcription factors, and to study TIGIT in our Scientific Poster Library.
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Cell Type: Tregs
Biology Area: Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Gene ID: NA
Regulatory Status: RUO
Other Names: Transcription Factor Fixation/Permeabilization Buffer Set