Biolegend, 427505, 2-Deoxy-D-glucose, VO27

2-Deoxy-D-glucose (2-DG) blocks glycolysis by inhibiting glucose-6-phosphate production. 2-DG is transported into the cell by glucose transporters where it is phosphorylated by hexokinase. Phosphorylated 2-DG cannot be further metabolized by phosphoglucose isomerase and accumulates in the cell, leading to the depletion of cellular ATP. 2-DG is used in metabolic studies to determine cellular dependency on glycolysis.
VO27
Formulation: Lyophilized
Preparation: Resuspend 1 g of lyophilized 2-Deoxy-D-Glucose in 60.9 mL of water or PBS to make a 100 mM stock solution. For 250 mg size, add 15.2 mL water or PBS to make a 100 mM stock solution.
Storage & Handling: Store lyophilized powder at -20°C. Once reconstituted, store aliquoted at -80°C. Use reconstituted material within 6 months to avoid loss of potency. Avoid freeze thaw cycles.
Application: Cell Culture - Verified
Recommended Usage: 2-Deoxy-D-glucose can be used for inhibition of glycolysis. For primary immune cells (e.g. PBMCs or splenocytes) a range of 2.5 – 10.0 mM is recommended. It is recommended that the reagent be titrated for each experiment to determine optimal performance.
Structure: C6H12O5. 164.16 g/mol
Function: Glycolysis inhibitor
Antigen References: Arguello, et al. 2020. Cell Metabolism. 32:1063-1075. Traba, et al. 2016. J Vis Exp. 117:54918. Li, et al. 2019. J Cell Phys. 234:11. Lin, et al .2017. Oncotarget. 8:110426. Repas, et al. 2022. Cancers. 14:1343.
Gene ID: NA
Regulatory Status: RUO
Other Names: Glycolysis Inhibitor, 2-Deoxyglucose, 2-Deoxy-D-arabinohexose, C6H12O5