Biolegend, 427508, Puromycin dihydrochloride, 50mg

Puromycin dihydrochloride is a naturally occurring amino nucleoside antibiotic produced by Streptomyces alboniger . Comprised of a nucleoside covalently bound to an amino acid, Puromycin mimics the 3′ end of aminoacylated tRNAs and thus inhibits protein synthesis by ribosome-catalyzed incorporation into elongating nascent chains, which results in premature termination of translation. It is used as a selection agent in mammalian cell cultures for cell lines that express a puromycin resistance via puromycin N-acetyl-transferase (pac). Puromycin immunodetection with antibodies such as BioLegend’s clone 2A4 allows the direct evaluation of translational activity in single cells by immunofluorescence microscopy and flow cytometry.
50mg
Formulation: Lyophilized
Preparation: Solubility: 50-100 mg/mL in distilled deionized water, PBS or DMSO.
Storage & Handling: Store lyophilized powder at -20°C. When reconstituted, aliquot and store at -20°C. Use reconstituted material within 6 months to avoid loss of potency.
Application: Cell Culture - Verified
Recommended Usage: Working concentrations of Puromycin dihydrochloride for cell-based assays range from 0.2 – 10 μg/mL. It is recommended that the reagent be titrated for each experiment to determine optimal performance.
Structure: C22 H29N7O5 · 2HCl. MW = 544.43 g/mol
Function: Protein synthesis inhibitor, antibiotic
Antigen References: Arguello R, et al. 2020. Cell Metabolism. 32:1063-1075. Nathans D, et al. 1964. PNAS.51:585-92. Miyamoto-Sato E, et al. 2000. Nucleic Acids Res. 28:1176-82. Schmidt EK, et al. 2009. Nat Methods. 6:275-7.
Gene ID: NA
Regulatory Status: RUO
Other Names: Protein Synthesis Inhibitor, Puromycin 2HCl, C22H31Cl2N7O5