Biolegend, 430523, FluoroSpot MAX™ Deluxe Set Human IL-6, 5plates

Human IL-6 (Interleukin), also known as B-cell Stimulatory Factor 2 (BSF2), Interferon beta-2 (IFN-β-2), Hybridoma Growth Factor (HGF), is a cytokine which is expressed by many different cell types such as macrophages, T-cells, osteoblasts, endothelial cells, and hepatocytes. It is involved in both pro-inflammatory and anti-inflammatory responses. In particular, IL-6 has critical roles in multiple different biological processes including but not limited to B-cell maturation, osteoclast formation, nerve cell differentiation, muscle healing and hypertrophy. Thus, abnormal level of expression and activity of IL-6 can result in a wide range of diseases from chronic inflammation, cancer, to neurodegenerative diseases. BioLegend’s FluoroSpot MAX™ Deluxe Set contains pre-optimized essential reagents, buffers, and solutions for the detection of human IL-6 secreting cells. In this assay, anti-human IL-6 capture antibodies are coated on a clear-bottom microplate. Cells (stimulated or unstimulated) are added to the wells and incubated, allowing secreted IL-6 to bind to nearby capture antibodies. After incubation, cells are removed, and biotinylated detection antibodies are added followed by Streptavidin-PE. The resulting red spots represent the “footprint” of an individual IL-6 -secreting cell, allowing for precise quantification of cytokine-producing cells in the sample.
5plates
Verified Reactivity: Human
Application: FluoroSpot
Cell Type: Endothelial cells, Macrophages, Osteoblasts, T cells
Biology Area: Cancer Biomarkers, Immuno-Oncology, Immunology, Innate Immunity, Neurodegeneration, Signal Transduction
Molecular Family: Cytokines/Chemokines, Innate Immune Signaling
Gene ID: 3569
UniProt: View information about IL-6 on UniProt.org
Regulatory Status: RUO
Other Names: Interleukin 6, B-cell Stimulatory Factor 2 (BSF-2), CTL Differentiation Factor (CDF), Hybridoma Growth Factor