Biolegend, 431807, LEGEND MAX™ Human IL-2 ELISA Kit, 1precoatedplate

Human IL-2 (also known as T-cell growth factor) is produced by T-cells in response to antigenic or mitogenic stimulation. IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells promoting proliferation and maturation. Additionally, IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendocytes. IL-2 is involved in treatment of cancers such as melanoma and renal cell cancer. BioLegend’s LEGEND MAX™ Human IL-2 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a Human IL-2 specific monoclonal antibody is pre-coated on a 96-well strip-well plate. BioLegend’s LEGEND MAX™ Human IL-2 ELISA Kit is specifically designed for the accurate quantitation of Human IL-2 from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive.
1precoatedplate
Verified Reactivity: Human
Application: ELISA
Product Citations: Gerace D, et al. 2023. Cell Rep Med. 4:100879. PubMed Yoon HJ, et al. 2022. Exp Mol Med. Online ahead of print. PubMed Qiu XM, et al. 2020. Reproduction. 251:159. PubMed
Sensitivity: 4 pg/mL
Standard Range: 15.6-1,000 pg/mL
Cell Sources: T cells
Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Gene ID: 3558
UniProt: View information about IL-2 on UniProt.org
Regulatory Status: RUO
Other Names: IL-2 Pre-coated ELISA Kit
Q: In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?
A: We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.
Q: I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?
A: The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.
Q: For some of your ELISA kits, why do my serum samples require dilution with assay buffer?
A: In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.