Biolegend, 434310, LEGEND MAX™ Human FGF-basic ELISA Kit, 5precoatedplates

Fibroblast growth factor-basic (FGF-basic) is a heparin-binding growth factor which belongs to a family of fibroblast growth factors (FGFs). FGF family members share 35-60% amino acid sequence homology and are highly conserved among species. FGF-basic has been isolated from neural, pituitary, adrenal cortical, and placental tissues. Human FGF-basic is a 17.2 kD protein containing 154 amino acids. Other isofoms (22, 22.5, 24 and 34 kD) have also been reported with distinct intracellular localizations and functions. FGFs are involved in many physiological and pathological processes including embryonic development, neuronal outgrowth, angiogenesis, and malignant transformation. FGF-basic is essential for the development and maintenance of vascular integrity during embryogenesis and is also a major angiogenic factor involved in wound healing, cardiovascular disease, and tumor neovascularization. BioLegend’s LEGEND MAX™ Human FGF-basic ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a human FGF-basic specific monoclonal antibody is pre-coated on a 96-well strip-well plate. BioLegend’s LEGEND MAX™ Human FGF-basic ELISA Kit is specifically designed for the accurate quantitation of human FGF-basic from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive.
5precoatedplates
Verified Reactivity: Human
Application: ELISA
Product Citations: Kriauciunaite K, et al. 2021. J Cell Physiol. 236:7642. PubMed
Sensitivity: 4 pg/mL
Standard Range: 12.5 - 800 pg/mL
Cell Sources: Brain, retina, pituitary, kidney, placenta, testis, corpus luteum, adrenal glands, monocytes, prostate, bone, liver, cartilage, endothelial cells, epithelial cells
Biology Area: Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family: Cytokines/Chemokines, Growth Factors
Gene ID: 2247
UniProt: View information about FGF-basic on UniProt.org
Regulatory Status: RUO
Other Names: Fibroblast growth factor 2 (Fgf-2), Fgfb, bFGF, FGF-basic Precoated ELISA Kit
Q: In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?
A: We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.
Q: I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?
A: The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.
Q: For some of your ELISA kits, why do my serum samples require dilution with assay buffer?
A: In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.