Biolegend, 438805, ELISA MAX™ Deluxe Set Human MCP-1/CCL2, 10plates

Please contact our sales representative for bulk pricing quotations. Human MCP-1, also known as CCL2, is a member of the CC β chemokine family. It is widely expressed in endothelial cells, smooth muscle cells, and monocytes in response to several atherogenic stimulants such as CD40 ligand, platelet derived growth factor (PDGF), interleukin-1β (IL-1β), and oxidized low density lipoprotein. Several recent in vivo studies have disclosed critical roles of MCP1 in atherosclerosis. In addition, MCP-1 has been implicated in monocytic infiltration of tissues during several inflammatory diseases, and has been implicated in macrophage-mediated tumor growth suppression in mice. MCP-1 has been shown to have direct effects on tumor cells in an autocrine and paracrine fashion in multiple cancers, including breast, lung, cervix, ovary, sarcoma, and prostate. In addition, MCP-1 plays a key role in the regulation of MMPs during transmigration. MCP-1 has been described as a new diagnostic marker and therapeutic target for progressive renal injury in diabetic nephropathy. Kidney epithelial cells, including glomerular podocytes and tubular cells, make MCP-1 in response to high glucose and advanced glycation end products. MCP-1 promotes inflammation and progressive injury in diabetic kidneys. Both the ELISA MAX™ Standard Set and ELISA MAX™ Deluxe Set are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.
10plates
Verified Reactivity: Human
Application: ELISA
Application References(PubMed link indicates BioLegend citation): Cho HY, et al. 2015. Cell Immunol. 293:95. PubMed
Product Citations: Cho H, et al. 2015. Cell Immunol. 293:95. PubMed Lee C, et al. 2017. Int J Mol Sci. 10.3390/ijms18071363. PubMed Veerasubramanian PK, et al. 2021. Bioengineering (Basel). 9:. PubMed Gril B, et al. 2018. Nat Commun. 9:2705. PubMed Zhao Q, et al. 2022. iScience. 25:105319. PubMed Bader A, et al. 2015. PLoS One. 10: 0138477. PubMed Li X, et al. 2022. Sci Rep. 12:17058. PubMed Skejoe C, et al. 2022. Am J Clin Exp Immunol. 11:34. PubMed
Sensitivity: 3.9 pg/mL
Standard Range: 7.8-500 pg/mL
Cell Sources: Endothelial cells, smooth muscle cells, and monocytes
Biology Area: Cell Biology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Gene ID: 6347
UniProt: View information about CCL2 on UniProt.org
Regulatory Status: RUO
Other Names: Monocyte chemotactic protein 1, chemokine (C-C motif) ligand 2, Ccl2, JE, Scya2, monocyte chemotactic and activating factor, MCAF
Q: Do the ELISA MAX™ Deluxe Sets come with plates?
A: No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.