Biolegend, 438808, LEGEND MAX™ Human MCP-1/CCL2 ELISA Kit, 5precoatedplates

Human MCP-1, also known as CCL2, is a member of the CC ß chemokine family. It is widely expressed in endothelial cells, smooth muscle cells, and monocytes in response to several atherogenic stimulants such as CD40 ligand, platelet derived growth factor (PDGF), interleukin-1ß (IL-1ß), and oxidized low density lipoprotein. Several recent in vivo studies have disclosed critical roles of MCP1 in atherosclerosis. In addition, MCP-1 has been implicated in monocytic infiltration of tissues during several inflammatory diseases, and has been implicated in macrophage-mediated tumor growth suppression in mice. MCP-1 has been shown to have direct effects on tumor cells in an autocrine and paracrine fashion in multiple cancers, including breast, lung, cervix, ovary, sarcoma, and prostate. In addition, MCP-1 plays a key role in the regulation of MMPs during transmigration. MCP-1 has been described as a new diagnostic marker and therapeutic target for progressive renal injury in diabetic nephropathy. Kidney epithelial cells, including glomerular podocytes and tubular cells, make MCP-1 in response to high glucose and advanced glycation end products. MCP-1 promotes inflammation and progressive injury in diabetic kidneys. The BioLegend LEGEND MAX™ Human MCP-1 ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a capture antibody. This kit is specifically designed for the accurate quantitation of human MCP-1 from cell culture supernatant, serum, plasma, and other biological fluids. This kit is analytically validated with ready-to-use reagents.
5precoatedplates
Verified Reactivity: Human
Application: ELISA
Application References(PubMed link indicates BioLegend citation): Li YF, et al. 2015. Nutrition. 31:691. PubMed
Product Citations: Xu C, et al. 2023. MedComm (2020). 4:e242. PubMed Shishkova D, et al. 2022. Int J Mol Sci. 23: . PubMed Rojas J, et al. 2017. Toxins. 10.3390/toxins9040125. PubMed Huang HW, et al. 2021. Int J Mol Sci. 22:. PubMed Gholamnezhadjafari R, et al. 2017. Innate Immun. 23:440. PubMed Li Y, et al. 2015. Nutrition. 31:691. PubMed Song J, et al. 2020. Mol Med Rep. 1.400694444. PubMed
Sensitivity: 1.6 pg/mL
Standard Range: 7.8-500 pg/mL
Cell Sources: Endothelial cells, smooth muscle cells, and monocytes
Biology Area: Cell Biology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Loberg RD, et al. 2007. Cancer Res. 67:9417 2. Gregory JL, et al. 2006. J. Immunol. 177:8072. 3. Qui Z, et al. 2009. Immunology. 214:835. 4. Reichel CA, et al. 2009. Arterioscler. Thromb. Vasc. Biol. ATVBAHA.109.193268v1. 5. McQuibban GA, et al. 2002. Blood 100:1160. 5. Tesh GH, et al. 2008. Am. J. Physiol. Renal. Physiol. 294:F697.
Gene ID: 6347
UniProt: View information about CCL2 on UniProt.org
Regulatory Status: RUO
Other Names: Monocyte chemotactic protein 1, chemokine (C-C motif) ligand 2, Ccl2, JE, Scya2, monocyte chemotactic and activating factor, MCAF
Q: In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?
A: We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.
Q: I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?
A: The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.
Q: For some of your ELISA kits, why do my serum samples require dilution with assay buffer?
A: In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.