Biolegend, 445705, ELISA MAX™ Deluxe Set Human IL-31, 10plates

Please contact our sales representative for bulk pricing quotations. Interleukin 31 (IL-31) is a newly identified member of the gp130/IL-6 cytokine family that is composed of 144 amino acid and has molecular mass of 16 kD. IL-31 is produced mainly by T helper type 2 cells. The mature form of the protein contains a four-helix structure. Human IL-31 shares 31% amino acid sequence identity with mouse IL-31. The BioLegend ELISA MAX™ Deluxe Set is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). A human IL-31 specific goat polyclonal antibody is first coated on a 96-well plate. Standards and samples are added to the wells, and IL-31 binds to the immobilized capture antibody. Next, a biotinylated goat polyclonal anti-human IL-31 detection antibody is added, producing an antibody-antigen-antibody “sandwich”. Avidin horseradish peroxidase is subsequently added, followed by TMB Substrate Solution, producing a blue color in proportion to the concentration of IL-31 present in the sample. Finally, the Stop Solution changes the reaction color from blue to yellow, and the microwell absorbance is read at 450 nm with a microplate reader.
10plates
Verified Reactivity: Human
Application: ELISA
Product Citations: Yaseen B, et al. 2020. Rheumatology (Oxford). 2625:59. PubMed Shin JY, et al. 2019. Exp Ther Med. 18:1914. PubMed
Sensitivity: 0.6 pg/mL
Standard Range: 37.5-2,400 pg/mL
Cell Sources: Helper T Cell
Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Gene ID: 386653
UniProt: View information about IL-31 on UniProt.org
Regulatory Status: RUO
Other Names: Interleukin-31, IL31 Precoated ELISA Kit
Q: Do the ELISA MAX™ Deluxe Sets come with plates?
A: No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.