Biolegend, 447907, LEGEND MAX™ Mouse IFN-α1 ELISA Kit, 1precoatedplate

IFN-α1 is a chemokine produced by dendritic cells, lymphocytes, macrophages and other cells in response to infection. It is expressed in the thymus, placenta, and the spleen, and is produced primarily by hematopoietic cells, in particular plasmacytoid dendritic cells found in the peripheral lymphoid organs. IFN-α1 binds to IFNAR, causing a cascade that promotes expression of IFN stimulated genes, which have anti-viral, anti-tumor, anti-proliferative, and immunomodulatory functions. The BioLegend LEGEND MAX™ Mouse IFN-α1 ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is precoated with a mouse monoclonal anti-mouse IFN-α1 antibody. The detection antibody is a biotinylated polyclonal anti-mouse IFN-α1 antibody. This kit is specifically designed for the accurate quantitation of mouse IFN-α1 in cell culture supernatant, serum and plasma. This kit is analytically validated with ready-to-use reagents.
1precoatedplate
Verified Reactivity: Mouse
Application: ELISA
Sensitivity: 1.67 pg/mL
Standard Range: 7.81 - 500 pg/mL
Cell Type: Macrophages, Monocytes
Biology Area: Immunology, Innate Immunity
Molecular Family: Cytokines/Chemokines
Gene ID: NA
UniProt: View information about IFN-alpha1 on UniProt.org
Regulatory Status: RUO
Other Names: IFNa, IFN-a1, IFN-alpha, IFNalpha, Interferon alpha, Interferon alpha-1, If, Ifa1, If1ai14
Q: In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?
A: We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.
Q: I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?
A: The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.
Q: For some of your ELISA kits, why do my serum samples require dilution with assay buffer?
A: In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.