Biolegend, 449507, LEGEND MAX™ Human IFN-β ELISA Kit, 1precoatedplate

IFN-β is a type I class of interferon produced by macrophages, dendritic cells, endothelial cells and primarily fibroblasts. It plays a significant antiviral activity role within the innate immune response to infection, cell differentiation and anti-tumor defenses. Once secreted, it binds to a homologous receptor complex and activates the Jak/STAT pathway, resulting in upregulating genes that inhibit viral infection and regulate MHC class 1 antigens. Multiple sclerosis is characterized by a deficiency of IFN beta 1 and is commonly been used as an injectable treatment.
1precoatedplate
Verified Reactivity: Human
Application: ELISA
Sensitivity: 0.91 ± 0.16 pg/mL
Standard Range: 2.34 - 150 pg/mL
Cell Type: B cells, Dendritic cells, Endothelial cells, Fibroblasts, Macrophages
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Proliferation and Viability, COVID-19, Innate Immunity, Signal Transduction
Molecular Family: Cytokines/Chemokines, Innate Immune Signaling
Gene ID: 3456
UniProt: View information about IFN-beta on UniProt.org
Regulatory Status: RUO
Other Names: Interferon beta 1
Q: In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?
A: We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.
Q: I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?
A: The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.
Q: For some of your ELISA kits, why do my serum samples require dilution with assay buffer?
A: In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.