Biolegend, 500501, Purified anti-human IL-3 Antibody, 50μg

IL-3 is a highly species-specific pleiotropic factor produced primarily by activated T cells though also by mast cells, keratinocytes, and astrocytes, which stimulates colony formation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures. The BVD8-3G11 antibody can neutralize the bioactivity of natural or recombinant IL-3.
50μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Yeast-expressed, recombinant human IL-3
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ELISA Capture - Quality testedWB, IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 0.5-2 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of human IL-3 protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: ELISA1-4 or ELISPOT Capture: The purified BVD8-3G11 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated BVD3-1F9 antibody (Cat. No. 500604) as the detecting antibody.Additional reported applications include: Western blotting4 and immunoprecipitation4.
Application References(PubMed link indicates BioLegend citation): Abrams JS, et al. 1992. Immunol. Rev. 127:5. (ELISA)Abrams JS et al. 1994. Eosnophil-active cytokines in human disease: development and use of monoclonal antibodies to il-3, il-5, and GM-CSF. In Eosinophils in Allergy and Inflammation. Gleich GJ and Kay AB, eds. Marcel Dekker, Inc. New York, NY. p. 133. (ELISA)Abrams J. 1995. Curr. Prot. Immunol. 6.20. (ELISA)Kaushansky K. 1992. J. Clin. Invest. 90:1879. (ELISA, IP, WB)Mahanty S, et al. 1992. J. Immunol. 148:3567.
RRID: AB_315103 (BioLegend Cat. No. 500502)
Structure: Cytokine; 14-30 kD (Mammalian)
Bioactivity: Proliferation/differentiation of almost all types of hematopoietic progenitors into granulocytes, macrophages, erythroid cells, megakaryocytes, mast cell colonies; induces expression of 20-α-steroid dehydrogenase, histidine, ornithine decarboxylase.
Cell Sources: Activated T cells, mast cells, eosinophils, keratinocytes, NK cells, endothelial cells.
Cell Targets: Erythroid cells, megakaryocytes, neutrophils, eosinophils, basophils, mast cells, monocytic lineages.
Receptors: IL-3R, α subunit (CD123), common β subunit (CDw131).
Biology Area: Cell Biology, Stem Cells
Molecular Family: Growth Factors, Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Frendl G. 1992. Int. J. Immunopharmacol. 14:421. 3. Ihle J. 1992. Chem. Immunology 51:65.
Regulation: Upregulated by T cell stimulation; downregulated by glucocorticoids.
Gene ID: 3562
UniProt: View information about IL-3 on UniProt.org
Clone: BVD8-3G11
Regulatory Status: RUO
Other Names: Interleukin-3, Burst promoting activity, Eosinophil colony stimulating factor (Eo-CSF), Hematopoietic cell growth factor (HCGF), Mast (MGF/MCGF), Multi-colony stimulating (Multi-CSF), P cell stimulating activity (PCSA), Thy1 inducing factor
Isotype: Rat IgG1, κ