Biolegend, 504201, Biotin anti-mouse IL-4 Antibody, 50μg

IL-4 is a pleiotropic cytokine produced by activated T cells, mast cells, and basophils. IL-4 is a potent lymphoid cell growth factor which stimulates the growth and activation of certain B cells and T cells. IL-4 is important for regulation of T helper subset development. The BVD6-24G2 antibody reacts with mouse interleukin-4 (IL-4). The BVD6-24G2 antibody can not neutralize the bioactivity of natural or recombinant IL-4.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: E. coli-expressed, recombinant mouse IL-4
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: ELISA Detection, ELISPOT Detection
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.25-1.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-4 recombinant protein ranging from 250 to 2 pg/ml are recommended for each ELISA plate. For use as an ELISPOT detection antibody, a concentration range of 1-4 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: ELISA Detection1-3,5,7,8 or ELISPOT Detection4: The biotinylated BVD6-24G2 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified 11B11 antibody (Cat. No. 504102/504108) as capture antibody and recombinant mouse IL-4 (Cat. No. 563201) as the standard. Additional reported applications (for the relevant formats) include: In Vivo Capture6. Note: For testing mouse IL-4 in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 431101 to 431106) are specially developed and recommended.
Application References(PubMed link indicates BioLegend citation): Abrams J, et al. 1992. Immunol. Rev. 127:5. Abrams J. 1995. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.20. Sander B, et al. 1993. J. Immunol. Meth. 166:201. Fujihashi K, et al. 1991. J. Immunol. Meth. 160:181. Sander B, et al. 1991. Immunol. Rev. 119:65.Finkelman F, et al. 2003. Curr. Prot. Immunol. John Wiley & Sons New York. Unit 6.28.Dzhagalov I, et al. 2007. J. Immunol. 178:2113.Xu G, et al. 2007. J. Immunol. 179:5358.Paveley RA, et al. 2011. Int J Parasitol. 41:1335. PubMed
Product Citations: Sato A, et al. 2022. Biol Pharm Bull. 45:1798. PubMed Lorente E, et al. 2016. Mol Cell Proteomics. 15: 2141 - 2151. PubMed Yan J, et al. 2020. Cell Rep. 107820:31. PubMed Paveley R, et al. 2011. Int J Parasitol. 41:1335. PubMed Xu G, et al. 2007. J Immunol. 179:5358. PubMed Phan TS, et al. 2021. Sci Adv. 7:. PubMed
RRID: AB_315323 (BioLegend Cat. No. 504201) AB_315324 (BioLegend Cat. No. 504202)
Structure: Cytokine; 15-19 kD (Mammalian)
Bioactivity: Differentiation of naïve CD4+ T cells to the TH2 type, proliferation/differentiation of activated B cells, expression of class II MHC antigens, and of low affinity IgE receptors in resting B cells
Cell Sources: Mast cells, T cells, bone marrow stromal cells
Cell Targets: B cells, T cells, monocytes, endothelial cells, fibroblasts
Receptors: Heterodimer IL-4Rα (CD124); γ-subunit (CD132) in common with IL-2R, IL-7R, IL-13R, IL-15R
Cell Type: Tregs
Biology Area: Cell Biology, Stem Cells
Molecular Family: Growth Factors, Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Boulay J, et al. 1992. Curr. Opin. Immunol. 4:294. 3. Dullens H, et al. 1991. In vivo 5:567. 4. Paul W. 1991. Blood 77:1859.
Regulation: Upregulated by IL-2, platelet activating factor; downregulated by TGF-β
Gene ID: 16189
UniProt: View information about IL-4 on UniProt.org
Clone: BVD6-24G2
Regulatory Status: RUO
Other Names: Interleukin-4, Ia inducing factor (IaIF), B cell stimulating factor-1 (BSF-1), Hodgkin's cell growth factor (HCGF), Mast cell growth factor-2 (MCGF-2), Macrophage fusion factor (MFF), T cell growth factor-2 (TCGF-2)
Isotype: Rat IgG1, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.