Biolegend, 504803, Biotin anti-mouse IL-9 Antibody, 50μg

IL-9 is a potent, T cell-derived, T cell growth factor which can also enhance mast cell activity and IL-3- or IL-4- dependent proliferation of bone marrow-derived mast cells. IL-9 synergizes with erythropoietin to promote erythroid colony formation. IL-9 induces high affinity IgE receptor expression and granzyme A and B in murine T helper clones. The D9302C12 antibody reacts with mouse IL-9. The D9302C12 antibody can neutralize the bioactivity of natural or recombinant mouse IL-9.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: Baculovirus-expressed, recombinant mouse IL-9
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: ELISA Detection - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.25-1.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-9 recombinant protein ranging from 4000 to 30 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: ELISA Detection1,2: The biotinylated D9302C12 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with Purified D8402E8 antibody (Cat. No. 504702) as the capture antibody.Neutralization2-5:The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IL-9 bioactivity (Cat. No. 504807 and 504808).Additional reported applications (for the relevant formats) include: Western blotting.
Application References(PubMed link indicates BioLegend citation): Abrams, J. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20. Renauld, J., et al. 1995. J. Leuk. Biol. 57:353. Sitkauskiene, B., et al. 2005. Respir Res. 6:33. Jones, T.G, et al. 2009. J Immunol. 183:5251-60. (Neut) PubMed Jones, T.G, et al. 2010. J. Immunol. 185:1804-11. (Neut) PubMed
Product Citations: Goswami R, et al. 2012. J Immunol. 188:968. PubMed Richard A, et al. 2015. J Immunol. 194:3567. PubMed
RRID: AB_315348 (BioLegend Cat. No. 504804)
Structure: Cytokine; 30-40 kD (Mammalian)
Bioactivity: Potentiates production of IgG, IgM, and IgE by IL-4-induced B lymphocytes; regulates granzyme family proteases; enhances proliferation of bone marrow mast cells with IL-3; production of IL-6 by mast cells
Cell Sources: IL-2 activated TH2 lymphocytes
Cell Targets: T lymphocytes, erythroid precursors
Receptors: IL-9R
Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Quesniaux, V. 1992. Research Immunology 143:385. 3. Renauld, J., et al. 1993. Adv. Immunol. 54:79. 4. Yang, Y. 1992. Leuk. Lymphoma 8:441.
Regulation: Upregulated by IL-1 after TCR activation, TNF-α; downregulated by antibodies to IL-2
Gene ID: 16198
UniProt: View information about IL-9 on UniProt.org
Clone: D9302C12
Regulatory Status: RUO
Other Names: Interleukin-9, P40, Mast cell growth factor (MCGF), Megakaryoblast growth factor, T cell growth factor-3 (TCGF-3)
Isotype: Armenian Hamster IgG
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.