Biolegend, 507502, Purified anti-human IFN-γ Antibody, 500μg

Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells. The 4S.B3 antibody reacts with the human IFN-γ. The MD-1 antibody reacts with human and monkey interferon-gamma (IFN-γ). The MD-1 antibody can neutralize the bioactivity of natural or recombinant IFN-γ.
500μg
Verified Reactivity: Human
Reported Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The solution should be stored undiluted between 2°C and 8°C.
Application: ELISA Capture - Quality tested ICFC, IHC-F, WB - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA Capture applications, the antibody should be titrated between 0.25 - 2 µg/ml to determine optimal condition. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: ELISA1,2,5 and ELISPOT3,4 Capture: The Purified MD-1 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 4S.B3 (Cat. No. 502504/502514) antibody as the detecting antibody and recombinant human IFN-γ (Cat. No. 570209) as the standard. The LEAF™ Purified antibody is suggested for ELISPOT capture. For ELISPOT capture applications, a concentration range of 0.5-2 µg/ml is recommended. Neutralization1: The LEAF™ Purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IFN-γ bioactivity (Cat. No. 507513). Additional reported applications (for the relevant formats) include: Western blotting1,2, intracellular staining for flow cytometry6, and immunohistochemical staining7 of paraformaldehyde-fixed, saponin-treated frozen tissue sections. Note: For testing human IFN-γ in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430101 to 430106) are specially developed and recommended.
Application References(PubMed link indicates BioLegend citation): Van der Meide P, et al. 1985. J. Immuno. Meth. 79:293. Yazdanbakhsh M, et al. 1993. Eur. J. Immuno. 23:3312. Besouw N, et al. 1994. J. Med. Primatol. 23:42. Van der Meide P, et al. 1995. J. Med. Primatol. 24:271. Hilkens C, et al. 1996. J. Immunol. 156:1722. Hamann D, et al. 1996. J. Immunol. 156:1387. Wassenaar A, et al. 1995. Infect. Immunity. 63:2147.
Product Citations: Posseme C, et al. 2022. Cell Rep. 39:110989. PubMed Haug V, et al. 2020. Transplantation. e260:104. PubMed Hess NJ, et al. 2020. J Immunol. 205:272. PubMed Huanosta-Murillo E, et al. 2021. Front Immunol. 12:668369. PubMed
RRID: AB_2122340 (BioLegend Cat. No. 507501) AB_2122339 (BioLegend Cat. No. 507502)
Structure: Cytokine; dimer; 20-25 kD (Mammalian)
Bioactivity: Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC
Cell Sources: CD8+ and CD4+ T cells, NK cells
Cell Targets: T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Receptors: IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Cell Type: Tregs
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321. 3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571. 4. Gray P, et al. 1987. Lymphokines 13:151.
Regulation: Upregulated by IL-2, FGF-basic, EGF; downregulated by vitamin D3 or DMN; labile at pH2
Gene ID: 3458
UniProt: View information about IFN-gamma on UniProt.org
Clone: MD-1
Regulatory Status: RUO
Other Names: Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma
Isotype: Mouse IgG1, κ