Biolegend, 512334, APC anti-human IL-17A Antibody, 100tests

IL-17A is the founding member of the IL-17 family, a group of six structurally related pro-inflammatory cytokines. IL-17A, secreted by activated CD4 + Th17 cell subpopulation, elicits multiple biological activities on a variety of cells including: the induction of IL-6, IL-8, G-CSF, and PGE2 production in epithelial, endothelial or fibroblasts; the enhancement of surface expression of ICAM-1 in fibroblasts; activation of NF-κB and costimulation of T cell proliferation. Recent studies demonstrated that, in mice, activated IL-17-secreting CD4 + helper T cells (Th17 cells) mediate an autoimmune arthritis that clinically and immunologically resembles rheumatoid arthritis (RA). Human IL-17A shows 63%, 63%, and 72% amino acid sequence identity to rat IL-17A, mouse IL-17A, and a protein encoded by the ORF13 gene of herpesvirus Saimiri (HVS), respectively.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant full length human IL-17A
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Product Citations: Rosenberg JM, et al. 2022. Med (N Y). 3:42. PubMed Wu X, et al. 2023. iScience. 26:106559. PubMed Shaw DG, et al. 2023. Nat Med. 29:1520. PubMed Nozawa K, et al. 2023. Front Immunol. 13:1084960. PubMed Lee YJ, et al. 2021. Front Immunol. 615369:12. PubMed Ogbechi J, et al. 2022. Front Immunol. 13:1001956. PubMed James E, et al. 2016. PLoS Pathog. 12: 1005375. PubMed Mutoh T, et al. 2020. Nat Commun. 11:1253. PubMed Su W, et al. 2022. Front Immunol. 13:952338. PubMed Zhan Y, et al. 2021. JCI Insight. 6:. PubMed Liang Y, et al. 2019. J Allergy Clin Immunol. 144:1025. PubMed Eken A, et al. 2020. North Clin Istanb. 0.513194444. PubMed Zhu Y, et al. 2019. Cell Stem Cell. 25:542. PubMed Martínez–Fábregas J, et al. 2019. Elife. 8:e49314. PubMed Martínez-Fábregas J, et al. 2020. Cell Rep. 33:108545. PubMed Maleki KT, et al. 2021. Cell Reports Medicine. 2(3):100220. PubMed Zhao Y, et al. 2020. Front Immunol. 2.572222222. PubMed Zhai Y, et al. 2018. Autophagy. 1.714583333. PubMed
RRID: AB_2563985 (BioLegend Cat. No. 512333) AB_2563986 (BioLegend Cat. No. 512334)
Structure: Cytokine; dimer; ~15 kD (Mammalian)
Bioactivity: Secretion of IL-6, IL-8, G-CSF, prostaglandin E2 by epithelial, endothelial or fibroblastic cells; stimulates cell migration, cord formation, and IL-6 secretion by stromal cells
Cell Sources: Activated T cell subpopulation
Cell Targets: Fibroblasts, epithelial and endothelial cells, stromal cells
Receptors: IL-17R
Cell Type: Tregs
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Hirota K, et al. 2007. J. Exp. Med. 204:41. 2. Furuzawa-Carballeda J, et al. 2007. Autoimmun. Rev. 6:169. 3. Witowski J, et al. 2007. Kidney Int. 71:514. 4. Gaffen SL, et al. 2006. Vitam. Horm. 74:255. 5. Hymowitz S, et al. 2001. EMBO J. 20:5332.
Gene ID: 3605
UniProt: View information about IL-17A on UniProt.org
Clone: BL168
Regulatory Status: RUO
Other Names: Interleukin IL-17A, IL-17, Cytotoxic T lymphocyte-associated antigen 8 (CTLA-8)
Isotype: Mouse IgG1, κ