Biolegend, 513006, Alexa Fluor® 647 anti-human IL-21 Antibody, 100tests

Interleukin 21 (IL-21) is a potent immunomodulatory cytokine primarily produced by NKT cells and CD4 + T cells, particularly the inflammatory Th17 subset. It exerts pleiotropic effects on both innate and adaptive immune responses. These include positive effects such as enhancing proliferation of NK cells and cytotoxic T cells, and inhibitory effects on the antigen-presenting function of dendritic cells. IL-21 also has proapoptotic effects on B cells and NK cells. Recent studies have shown that IL-21 functions as an autocrine cytokine, strongly inducing Th17 differentiation and suppressing Foxp3 expression, making it a potential therapeutic target for treating cancer and inflammatory diseases.
100tests
Verified Reactivity: Human
Reported Reactivity: Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant full length human IL-21
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Product Citations: Zhang H, et al. 2023. Nat Immunol. 24:96. PubMed Khanam A, et al. 2021. Front Immunol. 11:599648. PubMed Punik J, et al. 2021. Cell Reports. 35(13):109320. PubMed Shi S, et al. 2013. Microbes Infect. 15:697. PubMed
RRID: AB_1227662 (BioLegend Cat. No. 513005) AB_1227661 (BioLegend Cat. No. 513006)
Distribution: Th17
Function: Immunomodulation
Cell Sources: NK-T cells and T cells
Cell Targets: Cytotoxic T cells (CTL) and Natural Killer (NK) cells that can destroy malignant or infected cells
Receptors: IL-21R
Cell Type: Th17
Biology Area: Immunology, Innate Immunity
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Nurieva R. 2007. Nature 448:416. 2. Parrish-Novak J, et al. 2002. J. Leukocyte Biol. 72:856. 3. Dumoutier L, et al. 2000. Proc. Natl. Acad. Sci. USA 97:10144. 4. Asao H, et al. 2001. J. Immunol. 167:1. 5. Parrish-Novak J, et al. 2000. Nature 408:57.
Gene ID: 59067
UniProt: View information about IL-21 on UniProt.org
Clone: 3A3-N2
Regulatory Status: RUO
Other Names: Interleukin 21
Isotype: Mouse IgG1, κ