Biolegend, 515507, PerCP/Cyanine5.5 anti-RANTES (CCL5) Antibody, 25tests

RANTES (CCL5) is a member of the CC chemokine family. Its mature form consists of 68 amino acid residues. RANTES is a strong chemotractant for monocytes, NK cells, T cells, and eosinphils. RANTES may have important role in mediating immune and inflammatory responses.
25tests
Verified Reactivity: Human
Reported Reactivity: Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application References(PubMed link indicates BioLegend citation): Krensky AM, et al. 1997. Methods Enzymol. 287:162.
Product Citations: Zhang M, et al. 2023. JCI Insight. 8: . PubMed Wang F, et al. 2021. Cell Mol Gastroenterol Hepatol. 13:257. PubMed Tao P, et al. 2021. Sci Adv. 7:eabi6794. PubMed
RRID: AB_2562665 (BioLegend Cat. No. 515507) AB_2562666 (BioLegend Cat. No. 515508)
Structure: Human RANTES cDNA is a member of the C-C chemokine family with a MW of around 8 kD. Human and mouse RANTES have 85% homology.
Distribution: Reported to be produced by T cells, platelets, synovial fibroblasts, and some tumor cells.
Function: Able to chemoattract monocytes, NK cells, eosinophils, and T cells.
Interaction: Monocytes, NK cells, T cells, and eosinophils.
Cell Type: Fibroblasts, Platelets, T cells
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Von Luettichau. et al. 1996. Cytokine 8:89. 2. Schall TJ. 1991. Cytokine 3:165.
Gene ID: 20304
UniProt: View information about RANTES on UniProt.org
Clone: VL1
Regulatory Status: RUO
Other Names: CCL5, C-C motif chemokine 5, Small-inducible cytokine A5, T-cell-specific protein RANTES, SIS-delta, T cell-specific protein P228, TCP228, Eosinophil-chemotactic cytokine, EoCP
Isotype: Mouse IgG2b, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.