Biolegend, 515801, Biotin anti-mouse IL-1β Antibody, VO19

IL-1 refers to two proteins, IL-1α and IL-1β which are the products of distinct genes, but which are recognized by the same cell surface receptors. IL-1β is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses including the activation of B and T cells. The Poly5158 antibody reacts with the precursor and mature secreted forms of mouse IL-1β.
VO19
Verified Reactivity: Mouse
Antibody Type: Polyclonal
Host Species: Goat
Immunogen: Recombinant mouse IL-1β
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: ELISA Detection - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection, the suggested use of this reagent is 1:200 dilution. To obtain a linear standard curve, serial dilutions of IL-1β recombinant protein ranging from 2000 to 30 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: ELISA Detection: The biotinylated Poly5158 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with purified B122 antibody (Cat. No. 503502) as the capture antibody.
Additional Product Notes: This antibody can be used for 5 plates.
Product Citations: Morita M, et al. 2021. Heliyon. 7:e06228. PubMed Herrada AA, et al. 2022. Front Immunol. 13:841641. PubMed Zhao L, et al. 2021. STAR Protocols. 2:100732. PubMed Pahari S, et al. 2020. Autophagy. 1.375694444. PubMed
RRID: AB_2124615 (BioLegend Cat. No. 515801)
Structure: Cytokine; 17.5 kD (Mammalian)
Bioactivity: Stimulates T cells, B cells, proliferation/activation of NK cells, fibroblasts, thymocytes, glioblastoma cells, astroglia, microglia; monocyte transition from IL-1β to IL-1α when matured to macrophages
Cell Sources: Monocytes, tissue macrophages, Langerhan cells, dendritic cells, T lymphocytes, B lymphocytes, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma
Cell Targets: B cells, T cells, monocytes
Receptors: Type I IL-1R (CDw121a), Type II IL-1R (CDw121b)
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Bomford R, et al. Eds. 1989. Interleukin-1 inflammation and disease. Elsevier New York. 3. Brazel D, et al. 1991. Biotechnol. Ther. 2:241. 4. Dinarello C et al. 1996. Blood 87:2095.
Regulation: Upregulated by TNF-α, IFN-α, IFN-β, IFN-γ, bacterial endotoxins, viruses, mitogens, antigens; downregulated by IL-6, lipoproteins, lipids, and α2-macroglobulin
Gene ID: 3553
UniProt: View information about IL-1beta on UniProt.org
Clone: Poly5158
Regulatory Status: RUO
Other Names: Interleukin-1β, Catabolin, Hematopoietin-1 (H1), IFNβ-inducing factor, Interleukin-β, Osteoclast activating factor (OAF)
Isotype: Goat Polyclonal IgG
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.