Biolegend, 516003, Biotin anti-mouse/rat TNF-α Antibody, 50μg

TNF-α is secreted by macrophages, monocytes, neutrophils, T-cells (principally CD4+), and NK-cells. Many transformed cell lines also secrete TNF-α. Monomeric mouse TNF-α is 156 amino acid protein (N-glycosylated) with a reported molecular weight of 17.5 kD protein. TNF-α forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro , hemorraghic necrosis of tumors in vivo , increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.
50μg
Verified Reactivity: Mouse, Rat
Antibody Type: Polyclonal
Host Species: Goat
Immunogen: Recombinant mouse TNF-α
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: ELISA Detection - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.25 - 1.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of TNF-α recombinant protein ranging from 500 to 7.8 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
Additional Product Notes: ELISA Detection: The biotinylated Poly5160 antibody is useful as a detection antibody for a sandwich ELISA when used in conjunction with purified TN3-19.12 antibody (Cat. Nos. 506102 & 506106) as the capture antibody for detecting mouse and rat TNF-α. The biotinylated Poly5160 antibody can also be used as a detection antibody in a sandwich ELISA to detect mouse TNF-α when used in conjunction with purified MP6-XT22 (Cat. Nos. 506301 & 506302) and recombinant mouse TNF-α (Cat. No. 575209) as the standard. Note: For testing mouse TNF-α in serum, plasma or supernatant, BioLegend's LEGEND MAX™ Kits (Cat. Nos. 430907 & 430908) are specially developed and recommended.
Product Citations: Uchiyama A, et al. 2017. J Dermatol Sci. 10.1016/j.jdermsci.2017.02.285. PubMed Allen KD, et al. 2020. Connect Tissue Res. 61:82. PubMed Schwaderer J, et al. 2020. Cell Death Dis. 11:154. PubMed Zhao L, et al. 2021. STAR Protocols. 2:100732. PubMed
RRID: AB_2561411 (BioLegend Cat. No. 516003)
Structure: TNF superfamily; dimer/trimer; 18-150 kD (Mammalian).
Distribution: Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells.
Function: Type II integral membrane protein processed by TACE for secretion; upregulated by interferons, IL-2, GM-CSF, substance P, bradykinin, PAF, immune complexes, cyclooxygenase; downregulated by IL-6, TGF-β, vitamin D3, prostaglandin E2, PAF antagonists.
Interaction: Monocytes, neutrophils, macrophages, T cells, fibroblasts, endothelial cells, osteoclasts, adipocytes, astroglia, microglia.
Ligand/Receptor: TNFRSF1A (TNF-R1, CD120a, TNFR-p60 Type β, p55); TNFRSF1B (TNF-R2, CD120b, TNFR-p80 Type A, p75).
Cell Type: B cells, Macrophages, Monocytes, Neutrophils, NK cells, T cells
Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Beutler B, et al. 1988. Annu. Rev. Biochem. 57:505. 3. Beutler B, et al. 1989. Annu. Rev. Immunol. 7:625. 4. Tracey K, et al. 1993. Crit. Care Med. 21:S415.
Gene ID: 21926
UniProt: View information about TNF-alpha on UniProt.org
Clone: Poly5160
Regulatory Status: RUO
Other Names: Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF-2
Isotype: Goat Polyclonal IgG
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.