Biolegend, 516101, Purified anti-human IL-22 Antibody, 100μg

IL-22 is a cytokine structurally related to IL-10. Originally identified as a murine gene induced by IL-9 in T and mast cells, IL-22 was initially designated ILTIF (IL-10-related T cell-derived inducible factor).   IL-22 belongs to a family of cytokines with limited homology to IL-10, namely IL-10, IL-19, IL-20, IL-24, IL-26, and the IFN-λs, IL-28A, IL-28B and IL-29.  Human IL-22 shares 79% amino acid identity with murine IL-22 and 25% identity with human IL-10. IL-22 biological activity is initiated by binding to a cell surface complex composed of IL-22R1 and IL-10R2 receptor chains. Activity is further regulated through interactions with the soluble binding protein, IL-22BP, which shares sequence similarity with an extracellular region of IL-22R1 (sIL-22R1).  Both chains of the IL-22R complex belong to the class II CRF. Two types of IL-22 binding receptors have been discovered, a membrane-bound receptor and a soluble receptor, both encoded by different genes. IL-22 is produced by Th17 cells and newly identified Th22 cells. It plays a critical role in mucosal immunity in addition to the deregulated inflammation observed in autoimmune diseases.
100μg
Verified Reactivity: Human
Antibody Type: Polyclonal
Host Species: Goat
Immunogen: Human IL-22, amino acids Ala34-Ile179 (Accession# NM_020525) was expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by antigen affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: ELISA Capture - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA capture antibody, a concentration range of 1-4 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance. To obtain a linear standard curve, serial dilutions of IL-22 recombinant protein ranging from 8000-125 pg/ml are recommended.
Application Notes: ELISA Capture: The purified Poly5161 antibody is useful as a capture antibody in a sandwich ELISA assay, when used in conjunction with biotinylated Poly5161 antibody (Cat. No. 516103) as the detecting antibody and recombinant human IL-22 (Cat. No. 571309) as the standard.
RRID: AB_2561330 (BioLegend Cat. No. 516101)
Structure: Cytokine
Distribution: Thymic lymphomas, T cells, activated T cells, macrophages, and mast cells, Th17
Function: Enhances the production of acute phase proteins in a hepatocyte cell line, HepG2; Promotes Th17 cells to transmigrate through the blood-brain barrier in multiple sclerosis
Interaction: Interacts with a cell surface complex composed of IL-22R1 and IL-10R2 receptor chains which are expressed in epithelial cells of inflammatory diseases such as psoriasis, Crohn's disease, rheumatoid arthritis, or allergic asthma
Ligand/Receptor: IL-22R1 and IL-10R2 receptor chains
Cross: In sandwich ELISA, less than 10% cross-reactivity with recombinant mouse IL-22.
Cell Type: T cells, Macrophages, Th17, Mast cells
Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Nagalakshmi ML, et al. 2004. Intern. Immunopharmacol. 4:679. 2. Kebir H, et al. 2007. Nat. Med. 13:1173. 3. Gu Y, et al. 2008. Eur. J. Immunol. 38:1807. 4. Pene J, et al. 2008. J. Immunol. 180:7423. 5. Dumotier L, et al. 2000. J. Immune. 164:1814. 6. Xie MH, et al. 2000. J. Biol. Chem. 765:31335.7. Kotenko SV, et al. 2001. J. Immunol. 166:7096. 8. Chang C, et al. 2006. Cell Res. 16:902. 9. Trifari S,et al. 2009. Nat. Immunol. 10:864.
Gene ID: 50616
UniProt: View information about IL-22 on UniProt.org
Clone: Poly5161
Regulatory Status: RUO
Other Names: TIFa, IL-D110, IL-TIF, TIFIL-23, zcyto18
Isotype: Goat Polyclonal Ig