Biolegend, 517005, Alexa Fluor® 488 anti-mouse IL-17F Antibody, 25μg

Interleukin 17F (IL-17F) is a 37 kD IL-17 family member.  The IL-17 family consists of six members including IL-17 (also called IL-17A), IL-17B, IL-17C, IL-17D, IL-17E (also called IL-25), and IL-17F.  IL-17F shares the strongest similarity to IL-17A and forms a homodimer or heterodimer with IL-17A. It is produced by Th17 cells, mast cells, basophils, and epithelial cells.  IL-17F is an important regulator of inflammatory responses.  It is involved in host defense against mucoepithelial infection by Staphylococcus aureus and Citrobacter rodentium.  Over expression of IL-17F gene in the airway of mice is associated with airway neutrophilia, the induction of many cytokines, an increase in airway hyperreactivity, and mucus hyper-secretion. IL-17F is also involved in cancer immunity and autoimmune responses. IL-17F, like IL-17A, depends on IL-17R for its signaling in vitro and in vivo .  P38 MAPK, ERK1/2, Act1 (NF- K B activator protein 1), and TRAF6 are involved in IL-17F signaling.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Mouse IL-17F-OVA
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Application References(PubMed link indicates BioLegend citation): Obregon-Henao A, et al. 2013. PLoS One. 8:80669. PubMed
Product Citations: Obregón-Henao A, et al. 2013. PLoS One. 8:80669. PubMed Emgård J, et al. 2018. Immunity. 143:419. PubMed Ma S, et al. 2022. Cell Rep. 38:110520. PubMed Chong WP, et al. 2020. Immunity. 53(2):384-397.e5.. PubMed
RRID: AB_10660963 (BioLegend Cat. No. 517005) AB_10661903 (BioLegend Cat. No. 517006)
Structure: Forms a 37 kD homodimer or heterodimer with IL-17A of the IL-17 family member.
Distribution: Th17 cells, mast cells, basophils, and epithelial cells.
Function: An important regulator of inflammatory responses, involved in cancer immunity and autoimmune responses as well.
Ligand/Receptor: IL-17R (CD217)
Cell Type: Th17, Mast cells, Basophils, Epithelial cells
Biology Area: Immunology, Innate Immunity
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Dong C. 2008. Immunol. Rev. 226:80. 2. Kolls JK, et al. 2004. Immunity 21:467. 3. Aggarwal S, et al. 2002. J. Leukoc. Biol. 71:1. 4. Yang XO, et al. 2008. J. Exp. Med. 205:1063. 5. Ishigame H, et al. 2009. Immunity 30:108. 6. Hu Y, et al. 2010. J. Immunol. 184:4307.
Gene ID: 257630
UniProt: View information about IL-17F on UniProt.org
Clone: 9D3.1C8
Regulatory Status: RUO
Other Names: Interleukin-17F, ML-1,
Isotype: Mouse IgG1, κ