Biolegend, 517103, Alexa Fluor® 647 anti-mouse IL-17F Antibody, 25μg

Interleukin 17F (IL-17F) is a 37 kD IL-17 family member.  The IL-17 family consists of six members including IL-17 (also called IL-17A), IL-17B, IL-17C, IL-17D, IL-17E (also called IL-25), and IL-17F.  IL-17F shares the strongest similarity to IL-17A and forms a homodimer or heterodimer with IL-17A. It is produced by Th17 cells, mast cells, basophils, and epithelial cells.  IL-17F is an important regulator of inflammatory responses.  It is involved in host defense against mucoepithelial infection by Staphylococcus aureus and Citrobacter rodentium.  Overexpression of the IL-17F gene in the airways of mice is associated with airway neutrophilia, the induction of many cytokines, an increase in airway hyperreactivity, and mucus hypersecretion. IL-17F is also involved in cancer immunity and autoimmune responses. IL-17F, like IL-17A, depends on IL-17R for its signaling in vitro and in vivo .  P38 MAPK, ERK1/2, Act1 (NF-kB activator protein 1), and TRAF6 are involved in IL-17F signaling.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Mouse IL-17F-OVA
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: ELISA Detection: The biotinylated 8F5.1A9 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with purified TC11-18H10.1 (Cat. No. 506901 or 506906) antibody as the capture antibody and recombinant mouse IL-17A/F (Cat. No. 580809) as the standard. Flow Cytometry: The fluorochrome-labeled 8F5.1A9 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-17F-producing cells within mixed cell populations. Note: For testing mouse IL-17F in serum, plasma or supernatant, BioLegend's ELISA LEGEND MAX™ Kits (Cat. No. 436107 & 436108) are specially developed and recommended.
RRID: AB_2125412 (BioLegend Cat. No. 517104)
Structure: Forms a 37 kD homodimer or heterodimer with IL-17A, a IL-17 family member.
Distribution: Th17 cells, mast cells, basophils, and epithelial cells.
Function: An important regulator of inflammatory responses, involved in cancer immunity and autoimmune responses as well.
Ligand/Receptor: IL-17R (CD217)
Cell Type: Th17, Mast cells, Basophils, Epithelial cells
Biology Area: Immunology, Innate Immunity
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Dong C. 2008. Immunol. Rev. 226:80. 2. Kolls JK, et al. 2004. Immunity 21:467. 3. Aggarwal S, et al. 2002. J. Leukoc. Biol. 71:1. 4. Yang XO, et al. 2008. J. Exp. Med. 205:1063. 5. Ishigame H, et al. 2009. Immunity 30:108. 6. Hu Y, et al. 2010. J. Immunol. 184:4307.
Gene ID: 257630
UniProt: View information about IL-17F on UniProt.org
Clone: 8F5.1A9
Regulatory Status: RUO
Other Names: Interleukin-17F, ML-1
Isotype: Mouse IgG1, κ