Biolegend, 518001, Biotin anti-human C3a/C3a(desArg)/C3 Antibody, 50μg

C3, complement component 3, is a 195 KD glycoprotein heterodimer that is linked by disulfide bonds. It is the fourth complement component to react in the classical pathway and it is also a reactant in the alternative complement pathway. C3 convertase splits C3 into C3b and C3a. C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Macrophages and neutrophils have receptors for C3b. C3a is released into the surrounding fluids. It can bind to receptors on basophils and mast cells triggering them to release their vasoactive contents (e.g., histamine). Because of the role of these materials in anaphylaxis, C3a is called an anaphylatoxin. In blood, the C3a fragment is rapidly desarginated by serum carboxypeptidase N enzyme to generate C3a desArg. Both C3a and C3a desArg are not glycosylated.
50μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human C3a
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: ELISA Detection - Quality testedWB - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 0.5-2 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of C3a recombinant protein ranging from 39 to 2500 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The D17/1 antibody reacts with human C3a, C3a(desArg) and C3. The D17/1 antibody can neutralize the bioactivity of natural or recombinant C3a.ELISA Detection: The biotinylated D17/1 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the purified K13/16 antibody (Cat. No. 518102) as the capture antibody.
Application References(PubMed link indicates BioLegend citation): Oppermann M, et al. 1991. Kidney Int. 40:939947. Oppermann M, et al. 1992. Immunopharm. 24:119134. Elsner J, et al. 1994. Blood 83:3324.
RRID: AB_10896912 (BioLegend Cat. No. 518001) AB_10895750 (BioLegend Cat. No. 518002)
Ligand/Receptor: C3a bind to C3a receptor, C3a desArg bind to C5L2 (GPR77)
Biology Area: Cell Biology, Complement, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References: 1. de Bruijn MH, et al. 1985. P. Natl. Acad. Sci. USA 82:708. 2. Hugli TE, et al. 1975. J. Biol. Chem. 250:8293. 3. Oxvig C, et al. 1995. J. Biol. Chem. 270:13645.
Gene ID: 718
UniProt: View information about C3a/C3adesArg/C3 on UniProt.org
Clone: D17/1
Regulatory Status: RUO
Other Names: C3a, C3a desarg, C3
Isotype: Mouse IgG1, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.