Biolegend, 518202, Purified anti-human C5a/C5a(desArg) Antibody, 500μg

C5, complement component 5, is a protein involved in the complement system. It is cleaved into C5a and C5b by the C3/C5 convertase. The smaller fragment C5a is an anaphylatoxin and  is released into the fluid surroundings. C5a is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C5a also stimulates the locomotion of polymorphonuclear leukocytes (chemokinesis) and direct their migration toward sites of inflammation (chemotaxis). C5a contains 74 amino acids and is rapidly metabolised by a serum enzyme, carboxypeptidase B to a 73 amino acid form, C5a des-Arg. The major fragment C5b binds to the membrane initiating the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex.
500μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human C5a
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ELISA Capture - Quality testedWB - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 2-8 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of C5a recombinant protein ranging from 15.6 to 1000 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The C17/5 antibody reacts with human C5a and C5a(desArg). C17/5 is specific for a C5 activation-dependent neo-epitope expressed only on C5a and C5a(desArg) but not C5, and has been shown to be an effective inhibitor of C5a.4ELISA Capture: The purified C17/5 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated G25/2 antibody (Cat. No. 518306) as the detection antibody.
Application References(PubMed link indicates BioLegend citation): Oppermann M, et al. 1991. Kidney Int. 40:939947. Oppermann M, et al. 1992. Immunopharm. 24:119134. Elsner J, et al. 1994. Blood 83:3324. Wittmann M, et al. 1999. J. Immunol. 162: 6763.
Product Citations: Throm AA, et al. 2018. JCI Insight. :3. PubMed
RRID: AB_11123910 (BioLegend Cat. No. 518201) AB_11124718 (BioLegend Cat. No. 518202)
Biology Area: Cell Biology, Complement, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References: 1. Lundwall AB, et al. 1985. J. Biol. Chem. 260:2108. 2. Fernandez HN, et al. 1978. J. Biol. Chem. 253:6955. 3. Tack BF, et al. 1979. Biochemistry 18:1490.
Gene ID: 727
UniProt: View information about C5a/C5adesArg/C5 on UniProt.org
Clone: C17/5
Regulatory Status: RUO
Other Names: C5a, C5a desArg
Isotype: Mouse IgG1, κ