Biolegend, 518902, Biotin anti-human IL-17A Antibody, 50μg

IL-17A is the founding member of the IL-17 family, a group of six structurally related pro-inflammatory cytokines. IL-17A, secreted by activated CD4+ Th17 cell subpopulation, elicits multiple biological activities on a variety of cells including: the induction of IL-6, IL-8, G-CSF, and PGE2 production in epithelial, endothelial or fibroblasts; the enhancement of surface expression of ICAM-1 in fibroblasts; activation of NF-κB and costimulation of T cell proliferation. Recent studies demonstrated that, in mice, activated IL-17-secreting CD4+ helper T cells (Th17 cells) mediate an autoimmune arthritis that clinically and immunologically resembles rheumatoid arthritis (RA). Human IL-17A shows 63%, 63%, and 72% amino acid sequence identity to rat IL-17A, mouse IL-17A, and a protein encoded by the ORF13 gene of herpesvirus Saimiri (HVS), respectively.
50μg
Verified Reactivity: Human
Antibody Type: Polyclonal
Host Species: Goat
Immunogen: Recombinant human IL-17A, amino acids Ile20-Ala155 (Accession # NM_002190) was expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 1% BSA (USA origin) and 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: ELISA Detection - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.25-1 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance. To obtain a linear standard curve, serial dilutions of IL-17A recombinant protein ranging from 1000 to 15.6 pg/ml are recommended.
Application Notes: ELISA Detection: The biotinylated Poly5189 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with purified BL23 (Cat. No. 512702) antibody as the capture antibody and recombinant human IL-17A (Cat. No. 570509) as the standard.
Product Citations: Nicot S, et al. 2023. Sci Adv. eadi7838:9. PubMed
RRID: AB_2561347 (BioLegend Cat. No. 518902)
Structure: Cytokine; dimer; ~15 kD (Mammalian)
Bioactivity: Secretion of IL-6, IL-8, G-CSF, prostaglandin E2 by epithelial, endothelial or fibroblastic cells; stimulates cell migration, cord formation, and IL-6 secretion by stromal cells
Cell Sources: Activated T cell subpopulation
Cell Targets: Fibroblasts, epithelial and endothelial cells, stromal cells
Cross: In sandwich ELISA, cross-reactivity with human IL-17F is negligible and no cross-reactivity with mouse IL-17A is present.
Receptors: IL-17R
Biology Area: Cell Biology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Hirota K, et al. 2007. J. Exp. Med. 204:41.2. Furuzawa-Carballeda J, et al. 2007. Autoimmun. Rev. 6:169.3. Witowski J, et al. 2007. Kidney Int. 71:514.4. Gaffen SL, et al. 2006. Vitam. Horm. 74:255.5. Hymowitz S, et al. 2001. EMBO J. 20:5332.
Gene ID: 3605
UniProt: View information about IL-17A on UniProt.org
Clone: Poly5189
Regulatory Status: RUO
Other Names: Interleukin IL-17A, IL-17, Cytotoxic T lymphocyte-associated antigen 8 (CTLA-8)
Isotype: Goat Polyclonal Ig
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.