Biolegend, 519303, Biotin anti-mouse IL-34 Antibody, 50μg

IL-34 is a homodimeric glycoprotein which contains 241 amino acids. IL-34 is expressed in the heart, brain, lung, liver, kidney, thymus, spleen, and other organs. It specifically binds to CD115/M-CSF receptor (CSF-1 receptor or c-Fms) and activates ERK1/2 phosphorylation. The function of IL-34 overlaps with CSF-1 to regulate myeloid lineage differentiation, proliferation, and survival. Elevated levels of IL-34 are seen in the plasma of rheumatoid arthritis patients, but can be decreased in response to therapy. IL-34 levels are also increased in the serum of cancer patients and associated with poor prognosis. Mouse IL-34 shares 71% sequence identity with human IL-34 on the amino acid level.
50μg
Verified Reactivity: Mouse
Antibody Type: Polyclonal
Host Species: Goat
Immunogen: Recombinant mouse IL-34
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: ELISA Detection - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. A concentration of 0.5 µg/ml detection antibody was utilized to generate the example standard curve. It is recommended that the end-user titrate each lot of reagents for optimal performance.
Application Notes: ELISA Detection: To measure mouse IL-34, this antibody can be used as a detection antibody in sandwich ELISA format, and paired with the LEAF™ purified C054-35 antibody (Cat. No. 147201) as the capture antibody. Recombinant Mouse IL-34 (ELISA Std.) (Cat. No. 577609) can be used as the protein standard.Note: For testing mouse IL-34 in serum, plasma or cell culture supernatant, BioLegend's LEGEND MAX™ ELISA Kits (Cat. No. 439107 & 439108) are specially developed and recommended.
RRID: AB_2561419 (BioLegend Cat. No. 519303)
Structure: Homodimer
Distribution: Expressed in different tissues, including spleen, heart, brain, lung, liver, kidney, thymus, testes, ovary, small intestine, prostate, and colon
Function: Regulates myeloid lineage differentiation, proliferation, and survival
Interaction: Macrophages, monocytes, and myeloid cells
Ligand/Receptor: CD115/M-CSF receptor (CSF-1 receptor or c-Fms)
Cell Type: Dendritic cells
Biology Area: Immunology, Innate Immunity
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Lin H, et al. 2008. Sciences 320:807. 2. Chihara T, et al. 2010. Cell Death Differ. 17:1917. 3. Wei S, et al. 2010. J. Leuko. Biol. 88:495. 4. Eda H, et al. 2011. Rheumatol 31:1525. 5. Covaleda L, et al. 2010. Virology 397:217.
Gene ID: 76527
UniProt: View information about IL-34 on UniProt.org
Clone: Poly5193
Regulatory Status: RUO
Other Names: IL34, Interleukin 34, C16orf77
Isotype: Goat Polyclonal Ig
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.