Biolegend, 519502, Purified anti-human CXCL10 (IP-10) Antibody, 500μg

CXCL10, also known as IP-10, is a 8.7 kD chemokine. It belongs to the CXC chemokine family. It is expressed by monocytes, fibroblasts, and endothelial cells in response to IFNγ. It can interact with CXCR3 and plays an important role in immune cell trafficking, particularly of T cells, dendritic cells, and NK cells. In addition, it alters expression of adhesion molecules.
500μg
Verified Reactivity: Human
Reported Reactivity: Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ICFC - Quality tested WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Bengsch B et al. 2018. Immunity. 48(5):1029-1045 . PubMed Kang SH, et al. 2018. Oncoimmunology. 8:e1515057. PubMed
RRID: AB_2563205 (BioLegend Cat. No. 519501) AB_2563206 (BioLegend Cat. No. 519502)
Structure: CXC chemokine family, 8.7 kD
Distribution: Monocytes, endothelial cells, and fibroblasts
Function: Interacts with CXCR3 and serves as a chemoattractant for T cells, dendritic cells, monocytes/macrophages, and NK cells
Ligand/Receptor: CXCR3
Cell Type: B cells, Dendritic cells, Endothelial cells, Fibroblasts, Monocytes
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Luster AD, et al. 1987. Proc. Natl. Acad. Sci. USA 84:2868. 2. Kaplan G, et al. 1987. J. Exp. Med. 166:1098. 3. Sarris AH, et al. 1993. J. Exp. Med. 178:1127.
Gene ID: 3627
UniProt: View information about CXCL10 on UniProt.org
Clone: J034D6
Regulatory Status: RUO
Other Names: Interferon gamma induced protein-10 (IP-10)
Isotype: Mouse IgG2a, κ