Biolegend, 521603, LEAF™ Purified anti-human G-CSF Antibody, 100μg

Human G-CSF was cloned from a human squamous carcinoma cell line and is the key hematopoietic cytokine involved in the control of neutrophil production. Therefore, it is a critical regulator of innate immunity against bacterial infections. G-CSF mobilizes stem cells indirectly by downregulating the expression of CXCL12 on marrow osteoblasts and by releasing neutrophil and monocyte proteolytic enzymes, including neutrophil elastase, cathepsin G, and matrix metalloproteinase-9, which in turn degrades important HSC trafficking and adhesion molecules c-kit, VCAM-1, and CXCR4. In addition, G-CSF possesses immunosuppressive effects on monocytes/macrophages, dendritic cells, and T lymphocytes. The receptor for G-CSF is expressed not only in hematopoietic cells; it is also expressed in cardiomyocytes, skeletal muscle, and neurons. G-CSF influences mouse skeletal muscle development and regeneration by stimulating myoblast proliferation. In vitro , G-CSF displays strong antiapoptotic activity in neuronal cells.
100μg
Verified Reactivity: Human
Antibody Type: Polyclonal
Host Species: Goat
Immunogen: Recombinant human G-CSF
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.
Preparation: The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: Upon receipt, store frozen at -20°C. Make small volume aliquots if needed and avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
Application: Neut - Quality tested WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by neutralizing proliferation induced by recombinant human G-CSF (Cat. No. 578604) at 0.6 ng/mL on M-NFS-60 cells. ND50 range: 0.02 - 0.1 µg/mL. For western blotting, the suggested use of this reagent is 0.1 - 0.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody has not been tested for ICC.
RRID: AB_2927858 (BioLegend Cat. No. 521603)
Structure: Heterodimer
Distribution: Macrophages, monocytes, neutrophils, fibroblasts, endothelial cells, epithelial cells, and bone marrow stromal cells
Function: G-CSF acts on myeloid progenitor cells to stimulate survival, proliferation, and neutrophilic maturation; on mature neutrophils, G-CSF enhances survival, superoxido anion and alkaline phosphatase production, arachidonic acid release, and antibody dependent cellular cytotoxicity.
Interaction: Hematopoietic progenitor cells, neutrophilic granulocytes, monocytes, platelets, endothelial cells, trophoblastic cells, myoblasts, cardiomyocytes, cardiac fibroblasts, neurons, and some small cell lung carcinoma cell lines
Ligand/Receptor: CD114 (G-CSFR)
Antigen References: Nagata S, et al. 1986. Nature. 319:415. Kim HK, et al. 2006. Blood. 108:812. Donahue RE, et al. 2009. Blood. 114:2530. Shimoji K, et al. 2010. Cell Stem Cell. 6:227. Martins A, et al. 2010. IUBMB Life. 62:611. Hara M, et al. 2011. J Exp Med. 208:715.
Gene ID: 1441
UniProt: View information about G-CSF on UniProt.org
Clone: Poly21A06
Regulatory Status: RUO
Other Names: Granulocyte-Colony Stimulating Factor, CSF3, CSF-beta
Isotype: Goat Polyclonal IgG
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.