Biolegend, 525903, Biotin anti-mouse IL-27 Antibody, 50μg

IL-27, a heterodimer protein, belongs to the IL-12 cytokine family. IL-27 is composed of the Epstein-Barr virus-induced gene 3 (EBI-3), a 34 kD glycoprotein that is related to the p40 subunit of IL-12; and p28, a 28 kD glycoprotein that is related to the p35 subunit of IL-12 and p19 subunit of IL-23. IL-27 triggers expansion of antigen-specific naïve CD4 + T cells, promotes polarization of Th1 cells, and inhibits the development of Th2 and Th17 cells. It is known that IL-27 is an early product of activated antigen-presenting cells (APCs). IL-27 plays an important role in regulating the activity of B and T lymphocytes.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant mouse IL-27
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: ELISA Detection - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. A concentration of 0.125 µg/ml of the capture antibody was utilized to generate the example standard curve. It is recommended that the end-user titrate each lot of reagents for optimal performance.
Application Notes: ELISA Detection: To measure mouse IL-27, this antibody can be used as a detection antibody in sandwich ELISA format and paired with the purified MM27-7B1 antibody (Cat. No. 516902) as the capture antibody. Recombinant Mouse IL-27 (ELISA Std.) (Cat. No. 577409) can be used as the protein standard.Note: For measuring mouse IL-27 in serum, plasma or cell culture supernatant, LEGEND MAX™ Mouse IL-27 Heterodimer ELISA Kit with Pre-coated Plates (Cat. No. 438707 & 438708) are specially developed and recommended.
RRID: AB_2563423 (BioLegend Cat. No. 525903) AB_2563424 (BioLegend Cat. No. 525904)
Structure: A heterodimeric cytokine consisting of EBV-induced gene-3 (EBI3, an IL-12 p40-related protein) and p28 (a newly discovered IL-12 p35-related protein).
Distribution: Produced by antigen-presenting cells, including macrophages and dendritic cells.
Function: Drives rapid clonal expansion of naïve CD4+ T cells, promotes Th1 polarization and IFN-γ production; plays a role in the anti-inflammatory and antitumor reponses, and has potential in autoimmune disease therapy.
Interaction: T cells and NK cells.
Ligand/Receptor: IL-27R (TCCR) (WSX-1).
Cell Type: Antigen-presenting cells, Macrophages, Dendritic cells, B cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Salcedo R, et al. 2004. J. Immunol. 173:7170. 2. Owaki T, et al. 2005. J. Immunol. 175:2191. 3. Pflanz S, et al. 2002. Immunity 16:779. 4. Diveu C, et al. 2009. J. Immunol. 182:5748. 5. Morishima N, et al. 2005. J. Immunol. 175:1686. 6. Liu J, et al. 2007. J. Exp. Med. 204:141.
Gene ID: 246779
UniProt: View information about IL-27 on UniProt.org
Clone: B02P6E6
Regulatory Status: RUO
Other Names: Interleukin-27 p28, IL-27-A, IL-27 subunit alpha
Isotype: Rat IgG2a, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.