Biolegend, 526103, Biotin anti-human Granulysin Antibody, 50μg

Granulysin is a cytolytic protein present in granules of human natural killer (NK) cells and activated cytotoxic T lymphocytes (CTLs). Granulysin is synthesized as a 15 kD precursor, which is then cleaved at both the amino- and carboxy-termini into a 9 kD activated form. The 9 kD granulysin is released into the intercellular space between target and effector cells via a granule exocytosis pathway. The 9 kD granulysin is a chemoattractant for T lymphocytes, monocytes, and other inflammatory cells and activates the expression of a number of cytokines, including RANTES, MCP-1, MCP-3, MIP-1α, IL-1, IL-6, IL-10 and IFN-α. High serum levels of granulysin are associated with cytotoxic T cell and NK cell activity, and may thus represent a soluble biomarker for host-cellular immune responses. Serum granulysin has also been proposed as a biomarker for the Th1/Th2 balance. At the cellular level, granulysin may serve as a favorable prognostic marker for certain types of cancer.
50μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant 9 kD granulysin
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: ELISA Detection - Quality testedNeut, IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. A concentration of 1 µg/µl of the detection antibody was utilized to gernerate the example standard curve. It is recommended that the end-user titrate each lot of reagents for optimal performance.
Application Notes: ELISA Detection: To measure human granulysin, this antibody can be used as a detection antibody in sandwich ELISA format and paired with the purified DH2 antibody (Cat. No. 348007) as the capture antibody.Additional reported applications (for the relevant formats) include: neutralization1 and immunoprecipitation1.Note: For testing human granulysin in serum, plasma or cell culture supernatant, LEGEND MAX™ Human Granulysin ELISA Kit with Pre-coated Plates (Cat. No. 438007 & 438008) are specially developed and recommended.
Application References(PubMed link indicates BioLegend citation): Tewari P, et al. 2010. Blood. 116:3465. (Neut, IP)
RRID: AB_2563603 (BioLegend Cat. No. 526103) AB_2563604 (BioLegend Cat. No. 526104)
Structure: Saposin-like protein family of lipid binding protein, 9 kD and 15 kD
Distribution: NK cells, cytolytic T cells
Function: Cytolytic against bacteria, fungi, parasite and tumor cells; chemoattract inflammatory cells
Cell Type: Neutrophils, NK cells, T cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Hanson DA, et al. 1999. Mol. Immunol. 36:413. 2. Spada FM, et al. 2000. J. Exp. Med. 191:937. 3. Kalb ML, et al. 2012. J. Immunol. 188:1583.
Gene ID: 10578
UniProt: View information about Granulysin on UniProt.org
Clone: DH10
Regulatory Status: RUO
Other Names: GNLY, 519, LAG2, NKG5, LAG-2, D2S69E, TLA519
Isotype: Mouse IgG1, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.