Biolegend, 527003, LEAF™ Purified anti-mouse ACE2 Antibody, 100μg

ACE2 is a member of the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases. It catalyzes the cleavage of the decapeptide angiotensin I into angiotensin-(1-9) and angiotensin II (potent vasoconstrictor) into the vasodilator angiotensin-(1-7). ACE2 is a type I membrane protein that functions as a carboxypeptidase. It cleaves between a proline and a single hydrophobic/basic residue from the COOH-terminus of its substrates. The mouse full-length enzyme possesses 805 amino acids and the ECD includes amino acids 1–740. ACE2 is a zinc metalloprotease with considerable homology to angiotensin I-converting enzyme (ACE). Both enzymes contain the typical HEXXH zinc-binding motif, ACE has two catalytic sites and ACE2 has only one, and ACE2 is not inhibited by ACE inhibitors captopril, lisinopril, and enalaprilat. Studies in mice showed that disruption of ACE2 induced a severe cardiac contractility defect and increased angiotensin II levels in heart. Human ACE2 has been identified as the receptor for SARS (severe acute respiratory syndrome)-coronavirus. ACE2 binds to the coronavirus S protein present on the surface of the virion. Studies in SARS-CoV-ACE2 interactions showed that specific amino acids (Lys31 and Lys353) in human ACE2 were critical to virus-receptor binding, and naturally selected viral mutations in S1 (K479N and S487T) enhanced the affinity of S1 for human ACE2. Rat ACE2 is not a receptor for SARS-CoV and mouse ACE2 is a poor receptor. Rat ACE2 has two amino acids that do not favor the binding of coronavirus S protein, one of which is His353 that mouse ACE2 also possesses. Therefore, rat cells are not infected by SARS-CoV and mouse cells are infected inefficiently.
100μg
Verified Reactivity: Mouse, Human
Antibody Type: Polyclonal
Host Species: Goat
Immunogen: Recombinant mouse ACE2
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.
Preparation: The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: Upon receipt, store frozen at -20°C. Make small volume aliquots if needed and avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
Application: Block - Quality tested WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by inhibiting the cleavage of 10 µM Mca-YVADAPK(Dnp)-OH substrate by 25 ng mouse ACE2. The IC50 for this effect is 1.5 - 7.5 µg/mL. For western blotting, the suggested use of this reagent is 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2910466 (BioLegend Cat. No. 527003)
Structure: Monomer
Distribution: Vascular endothelial cells of the heart, kidney, brain, and testis
Function: Regulates renal and cardiovascular function
Interaction: Angiotensin I and angiotensin II are substrates for ACE2.
Ligand/Receptor: Receptor for SARS-CoV-2 entry
Cell Type: Monocytes
Biology Area: Angiogenesis, COVID-19, Immunology
Molecular Family: Enzymes and Regulators
Antigen References: Crackower MA, et al. 2002. Nature. 417:822-8. Vickers C, et al. 2002. J Biol Chem. 277:14838-43. Tikellis C, et al. 2003. Hypertension. 41:392-7. Guan Y, et al. 2003. Science. 302:276-8. Li F, et al. 2005. Science. 309:1864-8. Ferrario CM, et al. 2005. Am J Physiol Heart Circ Physiol. 289: H2281-90. Perlman S & Netland J. 2009. Nat Rev Microbiol. 7:439-50. Wu K, et al. 2011. J Virol. 85:5331-7. Ge XY, et al. 2013. Nature. 503:535-8. Letko M, et al. 2020. Nat Microbiol. 5:562-569.
Gene ID: 70008
UniProt: View information about ACE2 on UniProt.org
Clone: Poly5270
Regulatory Status: RUO
Isotype: Goat Polyclonal IgG
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.