Biolegend, 534502, Purified anti-human CCL20 (MIP-3α) Antibody, 500μg

CCL20 (MIP-3α) belongs to the CC chemokine family. Human CCL20 is a protein of 96 amino acids, which shares the highest homology of 28% amino acid identity with MIP-1β and 64% amino acid sequence identity with the mouse CCL20. CCL20 is produced by activated monocytes, T cells, neutrophils, endothelial cells, epithelial cells and fibroblasts. Its expression is induced by lipopolysaccharide (LPS) and inflammatory cytokines such as tumor necrosis factor, IL-1β and interferon-γ, and downregulated by IL-10. CCL20 is the ligand for chemokine receptor CCR6, which is expressed on memory T cells, B cells, and immature dendritic cells (DCs). Therefore, it is involved in the regulation of DC trafficking and recruitment and activation of T cells. CCL20 also has potent antimicrobial activity. Upregulation of CCL20 plays important roles in autoimmune processes. This uncludes pathologies of the central nerve system (MS), pathogenesis of encephalomyelitis, psoriasis, rheumatoid arthritis, and inflammatory bowel diseases. CCL20 is also involved in various cancers, such as gastrointestinal cancers, pancreatic cancer, hepatocellular carcinoma, and breast cancer. 500μg Verified Reactivity: Human Antibody Type: Monoclonal Host Species: Mouse Immunogen: In-house recombinant human CCL20 Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Preparation: The antibody was purified by affinity chromatography. Concentration: 0.5 mg/ml Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Application: ELISA Capture - Quality tested Recommended Usage: Each lot of this antibody is quality control tested by ELISA. For ELISA capture applications, a concentration of 2.0µg/mL is recommended. To obtain a linear standard curve, serial dilutions of human CCL20 (MIP-3α) recombinant protein ranging from 7.81pg/mL to 500pg/mL are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance. Application Notes: ELISA Capture: The purified L2412F11 antibody is useful as the capture antibody in a sandwich ELISA, when used in conjunction with the biotinylated L2418G3 antibody (Cat. No. 534604) as the detection antibody.Note: For testing human CCL20 (MIP-3a) in cell culture supernatant, serum, plasma (EDTA), and other biological fluids, BioLegend's LEGEND MAX™ Kits (Cat. No. 441407 & 441408) are specially developed and recommended. RRID: AB_2715819 (BioLegend Cat. No. 534502) Structure: Protein of 96 amino acid Distribution: Expression is induced by lipopolysaccharide (LPS) and inflammatory cytokines such as tumor necrosis factor, IL-1β and interferon-γ, and downregulated by IL-10. Function: Involved in the regulation of DC trafficking and the recruitment and activation of T cells. CCL20 also has potent antimicrobial activity. Ligand/Receptor: Ligand for chemokine receptor CCR6. Biology Area: Cell Biology, Signal Transduction Molecular Family: Cytokines/Chemokines Gene ID: 6364 UniProt: View information about CCL20 on UniProt.org Clone: L2412F11 Regulatory Status: RUO Other Names: CCL20, MIP-3ž€, MIP-3a, Chemokine (C-C motif) ligand 20, liver activation regulated chemokine, LARC, Macrophage Inflammatory Protein-3ž€, MIP-3-alpha Isotype: Mouse IgG2b, κ