Biolegend, 534802, Purified anti-human PlGF Antibody, 500μg

Placental growth factor (PlGF) is a member of the PDGF/VEGF family of growth factors. Four alternatively-spliced isoforms have been identified: PlGF-1, PlGF-2, PlGF-3, and PlGF-4. All isoforms are expressed in placental tissues, with PlGF-2 being expressed specifically during early placental development. PlGF-1 and PlGF-3 are considered diffusible isoforms, while PlGF-2 and PlGF-4 each contain a unique heparin binding domain that may increase associations with cell membranes. In addition to placental tissues, PlGF is expressed in the microvasculature, bone marrow, umbilical vein endothelia, uterine keratinocytes, and NK cells. PlGF binds to both VEGF-R1 and neuropilin, but not VEGF-R2 and induces monocyte activation, migration, inflammatory cytokine production. PlGF plays a key role in maternal vascular function during pregnancy, with circulating levels reaching a peak mid-gestation. It can be used as a marker for pregnancy-associated hypertensive disorders such as pre-eclampsia.
500μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant Protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ELISA-C - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA Capture applications, a concentration range of 1.0 - 4.0 µg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The purified 13C8 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated 24G3 antibody (Cat. No. 534904) as the detecting antibody.
RRID: AB_2894608 (BioLegend Cat. No. 534802)
Structure: Homodimer
Distribution: Vascular endothelial cells, smooth muscle cells, keratinocytes, hematopoitic cells, retinal pigment epithelial cells, tumor cells, and cerebellar stroma cells.
Function: PlGF is an angiogenic factor and induces proliferation and migration of endothelial cells. It stimulates endothelial cells and synergistically amplifies the action of VEGF. Soluble VEGFR1 (sFLT1) can regulate the accessibility of PlGF.
Interaction: Endothelial cells, Macrophages, and trophoblats
Ligand/Receptor: VEGFR1 (FLT-1) and neuropilin 1 (Nrp1)
Cell Type: Endothelial cells, Epithelial cells, Hematopoietic stem and progenitors
Antigen References: Maglione D, et al. 1991. Proc Natl Acad Sci USA. 88:9267. Carmeliet P, et al. 2001. Nat Med. 7:575. Casalou C, et al. 2007. Leukemia. 21:1590. Marcellini M, et al. 2006. Am J Pathol. 169:643. Yao J, et al. 2011. Proc Natl Acad Sci USA. 108:11590. Cai J, et al. 2011. PLoS One. 6:e18076. Sibiude J, et al. 2012. PLoS One. 7:e50208. Snuderl M, et al. 2013. Cell. 152:1065.
Gene ID: 5228
UniProt: View information about PlGF on UniProt.org
Clone: 13C8
Regulatory Status: RUO
Other Names: PlGF, PLGF, Placental Growth Factor
Isotype: Mouse IgG1, λ