Biolegend, 537102, Purified anti-human IFN-α2 Antibody, 500μg

Interferons are divided into type I, II, and III. Type I IFNs (IFN-α and IFN-β) are most abundant in number, distribution, and expression. Also, they are highly conserved among mammals in both structure and function. IFN-α2 has been used in the treatment of cancer such as bladder cancer, hepatocellular carcinoma, and leukemia. IFN-α2 augments the suppressed immune functions in patients with head and neck squamous cell carcinoma (HNSCC). IFN-α2 initiated T and NK cell mediated cytotoxicity of tumor cells through IFNγ dependent and independent mechanisms. IFN-α2 enhances suppressed T cell cytotoxicity by stimulation of the perforin-granzyme B system (IFNγ dependent). Also, IFN-α2 induces the expression of perforin-granzyme B in NK cells (NK mediated cytotoxicity, IFNγ independent). In a preliminary study, IFN-α2 appears to be an effective immunostimulator and impacts the clinical outcome in tongue squamous cell carcinoma patients. IFN-α had been used in the treatment of chronic hepatitis C (CHC); nevertheless, IFN-α is relatively unstable and requires frequent parenteral administration. Pegylation of IFN-α, polyethylene glycol (PEG)-IFN-α, reduces in vitro activity but increase the stability and plasma half-life of IFN-α; therefore, PEG-IFN-α has replaced IFN-α in CHC treatment.
500μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ELISA Capture - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA Capture applications, a concentration range of 2.0 - 8.0 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of IFN-α2 recombinant protein ranging from 12.5 to 800 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The pure M4509E12 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated BL25372 antibody (Cat. No. 537204) as the capture antibody and Recombinant Human IFN-a2 (ELISA Std.) (Cat. No. 592709)
RRID: AB_2783346 (BioLegend Cat. No. 537102)
Distribution: IFN-α is ubiquitously expressed.
Function: IFN-α stimulates cytotoxic T lymphocytes and natural killer cell function. IFN-α has immunomodulatory and antiviral properties. IFNα2a induces CXCL10 in human microvascular endothelial cells and pulmonary artery smooth muscle cells. It is induced by viral infection.
Interaction: A variety of cells express the IFNα receptor (IFNAR).
Ligand/Receptor: IFNAR complex has two components, IFNAR1 and IFNAR2.
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Soluble Receptors
Antigen References: Bose A and Baral R. 2007. Immunol Lett. 108:68. Zhao W, et al. 2008. J Immunol. 180:5483. Badiger R, et al. 2012. Plos One. 7:e46779. Aghemo A, et al. 2010. Nat Rev Gastroenterol Hepatol. 7:485. Becker-Merok A, et al. 2013. Lupus. 22:155. Mukherjee KK, et al. 2012. Indian J Med Res. 136:54.
Gene ID: 3440
UniProt: View information about IFN-alpha2 on UniProt.org
Clone: M4509E12
Regulatory Status: RUO
Other Names: IFN-alpha 2B, IFN-alphaA, IFNA2, IFNA2B, Interferon Alpha 2
Isotype: Mouse IgG1, κ