Biolegend, 571314, Cell-Vive™ GMP Recombinant Human IL-22 (carrier-free), 25μg

IL-22 is a cytokine structurally related to IL-10. It was originally identified in mouse as a gene induced by IL-9 in T cells and mast cells. IL-22 was initially designated as ILTIF (IL-10-related T cell-derived inducible factor). IL-22 belongs to a family of cytokines with limited homology to IL-10, namely IL-10, IL-19, IL-20, IL-24, IL-26, IL-28A, IL-28B and IL-29 (the later three also known as IFN-λ). Human IL-22 shares 79% amino acid identity with mouse IL-22 and 25% identity with human IL-10. IL-22 biological activity is initiated by the binding to a cell surface complex composed of IL-22R1 and IL-10R2 receptor chains and further regulated by interactions with a soluble binding protein, IL-22BP, which share sequence similarity with an extracellular region of IL-22R1 (sIL-22R1). Both chains of the IL-22R complex belong to the class II CRF. Two types of IL-22 binding receptors have been discovered, a membrane-bound receptor and a soluble receptor, both encoded by different genes. IL-22 is produced by immune cells and acts on nonimmune cells to regulate local tissue inflammation. As a product of T helper 17 lineage of CD4 (+) effector lymphocytes, IL-22 plays a critical role in mucosal immunity as well as in deregulated inflammation observed in autoimmune diseases. BioLegend Cell-Vive™ GMP Recombinant proteins are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12 in a dedicated GMP facility compliant with ISO 13485:2016. Specifications and processes include:
25μg
Source: Human IL-22, amino acids Ala34-Ile179 (Accession # NM_020525) was expressed in E. coli.
Molecular Mass: The 147 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 16 kDa. The DTT-reduced protein migrates at approximately 15 kDa and the non-reduced protein migrates at approximately 14 kDa by SDS-PAGE.
N-terminalSequence Analysis: Met-Ala-Pro-Ile-Ser-Ser-His-(Cys)-Arg-Leu
Purity: ≥ 95%, as determined by Coomassie stained SDS-PAGE
Formulation: 0.1 μm filtered protein solution is in 10 mM Sodium Phosphate buffer, 150 mM NaCl, pH 7.2.
Endotoxin Level: Less than or equal to 0.1 EU per μg of protein as determined by LAL method.
Residual Host Cell Protein Content: ≤ 1 ng/μg by ELISA
Concentration: 25 μg & 100 μg sizes are bottled at 0.2 mg/mL
Storage & Handling: Unopened vials can be stored at ≤ -70°C for 4 years, ≤ -20°C for 6 months, and between 2°C and 8°C for 2 weeks. Aliquots can be stored between -20°C to -70°C for 3 months and between 2°C and 8°C for 1 week. For maximum results, quick spin vial prior to opening. Stock solutions can also be prepared at 50 - 100 μg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Avoid repeated freeze/thaw cycles.
Activity: Cell-Vive™ GMP Recombinant Human IL-22 induces IL-10 production in human Colo205 cells in a dose-dependent manner. The ED50 for this effect is 0.06 – 0.42 ng/mL.
Application: Bioassay
Application Notes: BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected].
Application References(PubMed link indicates BioLegend citation): Dhiman R, et al. 2009. J. Immunol. 183:6639. Pubmed
Disclaimer: BioLegend Cell-Vive™ GMP Recombinant proteins are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.
Structure: Cytokine
Distribution: Thymic lymphomas, T cells, activated T cells, macrophages, and mast cells
Function: Enhances the production of acute phase proteins in a hepatocyte cell line, HepG2; Promotes Th17 cells to transmigrate through the blood-brain barrier in multiple sclerosis
Interaction: Interacts with a cell surface complex composed of IL-22R1 and IL-10R2 receptor chains which are expressed in epithelial cells of inflammatory diseases such as psoriasis, Crohn's disease, rheumatoid arthritis, or allergic asthma
Ligand/Receptor: IL-22R1 and IL-10R2 receptor chains
Bioactivity: IL-22 bioactivity was measured by its ability to induce IL-10 in human Colo205 cells
Antigen References: Nagalakshmi ML, et al. 2004. Int Immunopharmacol. 4:679-691. Kebir H, et al. 2007. Nat. Med. 13:1173-1175. Gu Y, et al. 2008. Eur. J. Immunol. 38:1807-1813. Pene J, et al. 2008. J. Immunol. 180:7423-7430. Dumotier L, et al. 2000. J immune. 164:1814-1819. Xie MH, et al. 2000. J. Biol. Chem. 765:31335-31339. Jones BC, et al. 2008. Structure. 16:1333-44. Kotenko SV, et al. 2001. J. Immunol. 166:7096-71033. Chang C, et al. 2006. Cell Res. 16:902-907.
Gene ID: 50616
UniProt: View information about IL-22 on UniProt.org
Other Names: IL22, IL-D110, IL-TIF, ILTIF, MGC79382, MGC79384, TIFIL-23, TIFa, zcyto18