Biolegend, 571809, Recombinant Human IL-4 (ELISA Std.), 4pack

IL-4 is a pleiotropic cytokine that is produced by activated T cells mast cells and basophils. IL-4 elicits many different biological responses, but has two dominant functions. The first is regulating differentiation of naïve CD4 + T cell to the Th2 type. Th2 cells produce IL-4, IL-5, IL-10 and IL-13, which tend to favor a humoral immune response while suppressing a cell-mediated immune response controlled by Th1 cells. The second is regulating IgE and IgG1 production by B cells. Recombinant human IL-4 is a 14.9 kD protein containing 129 amino acids.
4pack
Source: E. coli, approximately 15 kD
Formulation: Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors.
Endotoxin Level: Endotoxin level is less than 0.1 ng per µg (1EU/µg).
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: Unopened vials can be stored between 2°C and 8°C until the expiration date. Prior to use, reconstitute the lyophilized powder with 0.2 mL of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Avoid repeated freeze/thaw cycles.
Application: ELISA
Recommended Usage: Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 500 pg/ml to 4 pg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This IL-4 protein is useful as a standard for a human IL-4 sandwich ELISA, using unlabeled 8D4-8 antibody (catalog #500702) for capture and biotinylated MP4-25D2 antibody (catalog #500804) for detection.
Product Citations: Serre K, et al. 2011. PLoS One. 6:e20731. PubMed
Structure: Cytokine, 14.9 kD
Bioactivity: Differentiation of naïve CD4+ T cells to the TH2 type, proliferation/differentiation of activated B cells, expression of class II MHC antigens, and of low affinity IgE receptors in resting B cells
Cell Sources: Mast cells, T cells, bone marrow stromal cells
Cell Targets: B cells, T cells, monocytes, endothelial cells, fibroblasts
Receptors: Heterodimer IL-4Rα (CD124); γ-subunit (CD132) in common with IL-2R, IL-7R, IL-13R, IL-15R
Biology Area: Stem Cells, Immunology, Cell Biology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Boulay J, et al. 1992. Curr. Opin. Immunol. 4:294. 3. Dullens H, et al. 1991. In vivo 5:567. 4. Paul W. 1991. Blood 77:1859.
Regulation: Upregulated by IL-2, platelet activating factor; downregulated by TGF-β
Gene ID: 3565
UniProt: View information about IL-4 on UniProt.org
Regulatory Status: RUO
Other Names: Interleukin-4, Ia inducing factor (IaIF), B-cell stimulating factor-1 (BSF-1), Hodgkin's cell growth factor (HCGF), Mast cell growth factor-2 (MCGF-2), Macrophage fusion factor (MFF), T cell growth factor-2 (TCGF-2)