Biolegend, 600001, Purified anti-Lamin A/C Antibody, 25μg

Lamins A and C are structural components of the lamina, a scaffold of proteins localized to the nuclear inner membrane, and contribute to nuclear membrane dynamics and chromatin structure. Mutations in the LMNA gene have been linked to multiple laminopathy disorders, including Hutchinson-Gilford progeria syndrome (HGPS), an autosomal dominant disorder characterized by the appearance of accelerated and dramatic aging. Patients with HGPS have a mutation in the LMNA gene that results in aberrant splicing and production of the toxic, truncated Lamin A protein progerin that functions in a dominant negative pattern.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: GST fused to C-terminal fragment of Lamin A (amino acid residues 422-664)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per mL. For flow cytometric staining using our True-Phos™ Perm Buffer in Cell Suspensions Protocol, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: WL4G10 does not recognize mouse Lamin A or C. WL4G10 failed to immunoprecipitate Lamin A or C from HeLa lysates. During PD testing for ICC applications, WL4G10 staining of PFA-fixed HeLa cells was compatible with both Methanol and Triton X-100 permeabilization methods; observed staining was slightly better with Triton X-100 permabilization.
RRID: AB_2810655 (BioLegend Cat. No. 600001) AB_2810656 (BioLegend Cat. No. 600002)
Structure: Lamin A is a 664 amino acid protein with a predicted molecular weight of 74 kD. Lamin C is a 572 amino acid protein with a predicted molecular weight of 65 kD.
Distribution: Ubiquitously expressed/Nucleoplasmic side of inner nuclear membrane
Function: Fibrous component of nuclear lamina, provides framework for nuclear envelope, may interact with chromatin
Cell Type: Neurons
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers, Western Blot Controls
Molecular Family: Nuclear Markers, Organelle Markers
Gene ID: 4000
UniProt: View information about Lamin A on UniProt.org
Clone: WL4G10
Regulatory Status: RUO
Other Names: CMD1A, HGPS, LGMD1B, LMN1, LMNL1, MADA, PRO1
Isotype: Mouse IgG1, κ