Biolegend, 600552, Purified anti-AMPKα Antibody, 100μg

AMP-activated protein kinase alpha (AMPKα) is a subunit of the αβγ heterotrimeric AMPK protein complex. It is a key regulator of metabolism and energy homeostasis in eukaryotes through induction of catabolic pathways and deactivation of anabolic pathways. As an inhibitor of mammalian target of rapamycin complex 1 (mTORC1) pathway, AMPKα is involved in the regulation of cellular growth, cell cycle progression, autophagy, and may play a dual role as both tumor suppressor and promotor. AMPKα is activated via phosphorylation of Threonine residue 172 (Thr172) within the activation loop of the α subunit and is promoted by the allosteric binding of AMP and/or ADP to AMPK’s γ subunit. This phosphorylation is mediated by the serine/threonine kinase liver kinase B1 (LKB1) in conjunction with accessory subunits STRAD and MO25, and also by calcium/calmodulin-dependent protein kinase 2 (CAMKK2 aka CAMKKβ). Activation is induced by increases in the cellular AMP:ATP and ADP:ATP ratios  following a decline in ATP levels as well as by cellular stress, DNA damage, glucose starvation, and fluxes in calcium and nutrient levels. Localization of AMPKα (Thr172) at the spindle poles suggests a novel role for AMPK in mitotic spindle orientation. AMPKα is a key therapeutic target in the treatment of metabolic disorders and an emerging potential treatment target for cancer.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P, ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 0.5µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone was also tested for ICC using 4% PFA fixed HeLa cells, Triton X-100 or MeOH permeabilization, and produced mainly nuclear staining.
RRID: AB_2910471 (BioLegend Cat. No. 600551) AB_2910471 (BioLegend Cat. No. 600552)
Structure: AMPKα is a 559 amino acid protein with a predicted molecular weight of 64 kD.
Distribution: Heart, Skeletal muscle, Kidney / Nucleus and cytoplasm
Function: Serine/threonine-protein kinase, transcription regulation, Wnt signaling, metabolism
Ligand/Receptor: ATP-binding, magnesium, Metal-binding, Nucelotide-binding
Biology Area: Cell Biology, Signal Transduction
Molecular Family: Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References: Mihaylova MM, et al. 2011. Nat Cell Biol. 13:1016-23. Stein S, et al. 2000. Biochem J. 345:437-43. Thaiparambil JT, et al. 2012. Mol Cell Biol. 16:3203-17. Vara-Ciruelos D, et al. 2019. Open Biol. 9:190099. Willows R, et al. 2017. Biochem J. 474:3059-3073.
Gene ID: 5562
UniProt: View information about AMPKalpha on UniProt.org
Clone: 5C9-5B10-6D3
Regulatory Status: RUO
Other Names: PRKAA1, AMPKa1, AMPK, ACACA Kinase, Tau-Protein Kinase PRKAA1, Protein kinase AMP-Activated, Alpha 1 Catalytic Subunit , HMGCR Kinase
Isotype: Mouse IgG1, κ