Biolegend, 601201, Purified anti-TDRD3 Antibody, 25μg

Tudor Domain Containing 3 (TDRD3) is a protein which specially recognizes di-methylarginine containing proteins. As an effector molecular, it binds to histone methylarginine H3R17me2a and H4R3me2a marked by coactivator-associated arginine methyltransferase (CARM1/PRMT4). In addition, TDRD3 recognizes the Arg1810 me2a modification in the CTD of RNA polymerase II, therefore is associated with transcriptional activation. Recent study suggests other roles other than effector in the nucleus. In cytoplasm, TDRD3 interacts with ubiquitin-specific protease 9 X-linked (USP9X) in cytoplasmic stress granules and is suggested that TDRD3 might regulate USP9X de-ubiqutinase activity. Knockdown of TDRD3 in breast cancer cells sensitizes chemotherapeutic drug-induced apoptosis.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial human TDRD3 (402-651 a.a.) recombinant protein expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 2.0 µg per ml (1:250 - 1:2500 dilution). For immunocytochemistry, the recommended usage is 0.5 - 2.0 µg per ml (1:250 - 1:1000 dilution). It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody does not cross-react with mouse (in-house tested).
RRID: AB_2715902 (BioLegend Cat. No. 601201) AB_2715903 (BioLegend Cat. No. 601202)
Structure: 651 amino acids with a predicted molecular weight of 73 kD. Contains a C-terminal Tudor domain responsible for recognizing symmetrically dimethylated arginine.
Distribution: Cytoplasm, nucleus.
Function: TDRD3 recognizes di-methylarginine containing proteins and functions as a transcriptional activator.
Interaction: TDRD3 interacts with FMR1, FXR1, FXR2, EWSR1, FUS, SERBP1, EEF1A1, DDX3, POLR2A, SNRPB, and SNRPN.
Biology Area: Cell Biology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Goto-Ito S, et al. 2017. Sci Rep. 7:42123. 2. Narayanan N, et al. 2017. Cell Discov. 3:16048. 3. Sikorsky T, et al. 2012. Nucleic Acids Res. 40:11748. 4. Yang Y, et al. 2010. Mol Cell. 40:1016. 5. Linder B, et al. 2008. Hum Mol Genet. 17:3236. 6. Goulet I, et al. 2008. Hum Mol Genet. 17:3055.
Gene ID: 81550
UniProt: View information about TDRD3 on UniProt.org
Clone: W16174A
Regulatory Status: RUO
Other Names: Tudor domain-containing protein 3
Isotype: Rat IgG2a, κ