Biolegend, 601607, Alexa Fluor® 647 anti-human Cytokeratin 7 Antibody, 25μg

Keratin 7 (KRT7) belongs to type II keratin and is expressed in the epithelia lining the cavities of internal organs, gland ducts and blood vessels. The function of KRT7 is still not clear but it has been suggested involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). In cancer, the expression of KRT7 is observed in many types of carcinoma and is associated with neoplasm progression. The diverse and unique expression pattern of KRT7 is often used as a diagnosis indicator in epithelial origin carcinoma. For example, aberrant KRT7 expression is observed in triple negative breast cancer. In bladder cancer, loss of KRT7 results in promoting proliferation of the bladder urothelium.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Human KRT7 peptide (446-462) conjugated to KLH.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.5 - 2.0 μg/ml (1:250-1:1000 dilution) is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: This antibody does not react with mouse (in-house tested). Additional reported applications (for the relevant formats) include: spatial biology (IBEX)1,2.
Application References(PubMed link indicates BioLegend citation): Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
RRID: AB_2728460 (BioLegend Cat. No. 601607) AB_2728461 (BioLegend Cat. No. 601608)
Structure: Type II cytokeratin. 469 amino acids with a predicted molecular weight of 51 kD.
Distribution: Cytoplasm.
Function: KRT7 is a type II keratin, involved in the translational regulation of the human papillomavirus type 16 E7 mRNA.
Interaction: KRT7 interacts with EIF3S10, HPV16 E7, and GPER1.
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family: Intermediate Filaments
Antigen References: 1. Tajima Y, et al. 2017. Sci Rep. 7:40684. 2. Huang B, et al. 2016. Oncogene. 35:4927. 3. Szponar A, et al. 2012. Virchows Arch. 460:423. 4. Sano M, et al. 2010. Int J Oncol. 36:321.
Gene ID: 3855
UniProt: View information about Cytokeratin 7 on UniProt.org
Clone: W16155A
Regulatory Status: RUO
Other Names: Keratin type II cytoskeletal 7, Cytokeratin-7, CK-7, Keratin-7, K7, Type-II keratin Kb7, Sarcolectin, SCL, Cytokeratin 7, Keratin 7
Isotype: Rat IgG2a, κ