Biolegend, 601754, Ultra-LEAF™ Purified anti-human CCL11 (Eotaxin) Antibody, 1mg

CCL11 is a member of the CC chemokine family and was originally identified as a chemoattractant for eosinophils. In response to allergens, CCL11, which participates in a variety of allergic diseases, promotes accumulation of eosinophils. In addition, CCL11 facilitates proliferation and metastasis of tumor cells expressing the CCL11 receptor, CCR3. CCL11 has been shown to induce angiogenic response by CCR3-positive microvascular endothelial cells.
1mg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human CCL11 (Eotaxin)
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: Neut - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by neutralizing the chemotaxis induced by recombinant human CCL11 (Eotaxin) (Cat. No. 583002) on mouse Baf3-mCCR3 transfectant chemoattractant cells. ND50 range: 0.1 - 0.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2922630 (BioLegend Cat. No. 601753) AB_2922630 (BioLegend Cat. No. 601754)
Structure: Chemokine
Distribution: Epithelial cells, smooth muscle cells, dendritic cells, and fibroblasts
Function: CCL11 selectively recruits eosinophils through binding to the chemokine receptor CCR3 during allergic response. CCL11 production is upregulated in response to several proimflammatory cytokines, such as IL-4, IL-13, TNF-α, and IFN-α, produced by mast cells and Th2 lymphocytes. CCL11 has been reported to stimulate eosinophil release from bone marrow, resulting in an elevated level of circulating eosinophils.
Interaction: Eosinophils
Ligand/Receptor: CCR3
Cell Type: Dendritic cells
Biology Area: Angiogenesis, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: Conroy DM, et al. 2001. Respir Res. 2:150-6. Levina V, et al. 2009. Clin Cancer Res. 15:2647-56. Salcedo R, et al. 2001. J Immunol. 166:7571-8. Kaehler J, et al. 2006. J Investig Med. 54:446-54. Hadjicharalambous C, et al. 2004. J Allergy Clin Immunol. 113:657-62. Crump MP, et al. 1998. J Biol Chem. 273:22471-9.
Gene ID: 6356
UniProt: View information about CCL11 on UniProt.org
Clone: A16056E
Regulatory Status: RUO
Other Names: Eotaxin, eotaxin-1
Isotype: Mouse IgG2a, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.