Biolegend, 603351, Purified anti-RIP-1 Antibody, 25μg

Receptor-interacting serine/threonine-protein kinase 1 (RIP-1) is a member of the RIP family of serine/threonine kinases and consists of a kinase domain (KD) at its N-terminus, and a death domain (DD) in its C-terminus. It is a key regulator of cellular inflammatory response and cell survival when functioning as a scaffold protein, namely as part of the NF-κB signaling pathway, and is a key regulator of cell death when functioning as a kinase. RIP-1 promotes cell survival as part of the tumor necrosis factor receptor 1 (TNFR1) complex following ubiquitination by M1 and K63, and phosphorylation mediated by IKK. When ubiquitination is disrupted by ubiquitin E3 ligases, RIP-1 associates with FADD and caspase 8 to promote apoptosis or forms a complex with RIPK3 to induce necroptosis. Due to it being part of the TNF signaling pathway and its dual roles in cell survival and cell death, RIP-1 is of particular interest as a therapeutic target, namely as a mediator in the induction of immunogenic cell death and the treatment of cancer as well as in the treatment of autoimmune and neurodegenerative disorders.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human RIP1 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 µg/mL. For immunocytochemistry, a concentration of 5 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone was tested for ICC using HeLa cells with localization expected in the cytosol and plasma membrane. Three fix/perm methods were used: methanol fixation, or 4% PFA fixation followed by permeabilization with either methanol or Triton X-100. Methanol-only fixation produced a strong signal with the expected cytoplasmic localization. PFA-fixation followed by permeabilization with either methanol or Triton X-100 did not produce the expected cytoplasmic/membrane signal. This clone was tested for IHC-P on human skin tissue using Biolegend’s AEC Chromagen. Positive signal was not observed.
RRID: AB_2922634 (BioLegend Cat. No. 603351) AB_2922634 (BioLegend Cat. No. 603352)
Structure: RIP-1 is a 671 amino acid protein with a predicted molecular weight of 76 kD.
Distribution: Ubiquitously expressed/localized to the cell membrane and cytoplasm
Function: Serine/threonine-protein kinase, Transferase
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Death, Neurodegeneration, Neuroscience
Molecular Family: Protein Kinases/Phosphatase
Antigen References: Ang RL, et al. 2019. Front Cell Dev Biol. 7:163. Degterev A, et al. 2019. Proc Natl Acad Sci U S A. 116:9714-22. Moriwaki K, et al. 2021. Mucosal Immunol. 15:84-95. Smith HG, et al. 2020. EMBO Mol Med. 12:e10979.
Gene ID: 8737
UniProt: View information about RIP-1 on UniProt.org
Clone: W17101A
Regulatory Status: RUO
Other Names: Receptor-Interacting Protein Kinase 1, RIP, RIP-1, RIP1, IMD57, AIEFL, Receptor-Interacting Serine/Threonine-Protein Kinase
Isotype: Rat IgG2b, λ